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Distribution of the rare earth elements and the modification of calcium concentrations in organs of mice administered with chloride compounds of the rare earth elements

施用稀土元素氯化物的小鼠器官中稀土元素的分布和钙浓度的变化

基本信息

批准号：
02670227
负责人：
SHINOHARA Atsuko
金额：
$ 0.19万
依托单位：
Juntendo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

项目摘要

The rare earth elements are being used in the field of electronics, super conductors, alloys, nagnetic materials. and luminous materials. In order to investigate biological effects of rare earth elements distribution of these elements in organs of mice was observed after administration of rare earth compounds. The effects of the administration of these elements on the concentrations of biologically essential elements such as Ca. Mg. Zn. Fe. and P in organs was also observed.1. Quantitative methods for determination of yttrium(Y) and lanthanide elements in biological materials were developed. After dissolving organs of mice by simple wetwashing procedure, Eu. Tb, or Dy complex with 2.6-pyridinedicarboxylic acid was measured by fluorometry. The detection limit of each element in this method was ; I ng/mi in Eu, I ng/ml in Tb, or 5 ng/ml in Dy.2. All of the elements used (Y. La, Ce, Nd, Sm. Eu, Gd, Tb, Dy. Ho, Er, and Yb) were available to determine using ion chlomatography combined with … More post-column reaction using arsenazo-III solution. The detection limit of this method was 2.5 ng in each element. Sensitivities for Eu, Tb, and Dy were increased 5-20 fold by using 2, 6-pyridinedicarboxilic acid solution as post-column reaction reagent in comparison with arsenazo-III solution.3. The concentrations of Y or lanthanides in various organs of mice were determined after intravenous administration of their chloride compounds (25 mg element/kg body weight). Twenty hours after the administration, high concentrations of elements administered were found in spleen, lung, and liver. Similar organ distribution was found in mice administered 10 mg/kg Eu, Tb, of Dy. By the simultaneous administration of Eu, Tb, and Dy at 10 mg /kg each almost equql amounts of these elements were incorporated in spleen, as well as in lung and liver.4. The increase of Ca concentration was clealy observed in spleen, lung, and liver in which high concentrations of administered elements were found. No significant changes in Mg, Zn, Fe, and P concentrations by the administration with the rare earth elements were observed in all organs tested.5. The concentrations of administered elements and increase ratio of Ca concentration (administered/control) were different depending on the organ and element used. The relation between these changes and chemical or physical properties of rare earth elements, however, was not clear. Less

稀土元素在电子、超导、合金、磁性材料等领域有着广泛的应用。和发光材料。为探讨稀土元素的生物学效应，观察了稀土化合物给药后在小鼠脏器中的分布。这些元素的管理上的生物必需元素，如钙的浓度的影响。Mg. Zn.菲。各器官中P含量也有所降低.建立了生物样品中钇和镧系元素的定量分析方法。采用简单的湿洗法溶解小鼠脏器后，Eu. Tb、Dy与2.6-吡啶二甲酸的配合物用荧光法测定。该方法中各元素的检出限为：Eu为1 ng/ml，Tb为1 ng/ml，Dy为5 ng/ml。所有使用的元素（Y。La、Ce、Nd、Sm。Eu、Gd、Tb、Dy。Ho、Er和Yb）可使用离子色谱法结合 ...更多信息使用偶氮胂-III溶液的柱后反应。该方法的检出限为2.5 ng。用2，6-吡啶二甲酸溶液作柱后反应试剂，Eu、Tb、Dy的灵敏度比偶氮胂-Ⅲ溶液提高5-20倍.静脉注射氯化物（25 mg元素/kg体重）后，测定小鼠各器官中Y或镧系元素的浓度。给药后20小时，在脾、肺和肝中发现了高浓度的给药元素。在给予10 mg/kg Eu、Tb、Dy的小鼠中发现类似的器官分布。同时给予Eu、Tb和Dy各10 mg /kg时，脾、肺和肝中几乎等量地掺入了这些元素.在高浓度稀土元素组，Ca含量明显增加的器官有脾、肺和肝，而Mg、Zn、Fe和P含量无明显变化.施用元素的浓度和Ca浓度的增加比率（施用/对照）根据所使用的器官和元素而不同。然而，这些变化与稀土元素的化学或物理性质之间的关系尚不清楚。少