Distribution of the rare earth elements and the modification of calcium concentrations in organs of mice administered with chloride compounds of the rare earth elements

稀土元素被应用于电子、超导体、合金、磁性材料等领域。还有发光材料。为了研究稀土元素的生物学效应，观察了稀土化合物给药后小鼠各器官中稀土元素的分布情况。这些元素的施用对生物必需元素如钙、镁浓度的影响。锌。菲。P在各脏器中的含量也有所变化。建立了生物材料中钇和镧系元素的定量测定方法。在用简单的湿洗程序溶解小鼠器官后，用荧光法测定了与2.6-吡啶二羧酸配合物Tb、Dy的含量。本方法中各元素的检出限为；Eu是1 ng/ml， Tb是1 ng/ml， dy2是5 ng/ml。所有使用的元素(Y. La, Ce, Nd, Sm。Eu, Gd, Tb, Dy, Ho, Er, Yb)均可通过离子层析法测定。每种元素的检出限为2.5 ng。与偶氮胂溶液相比，2,6 -吡啶二羧酸溶液作为柱后反应试剂，对Eu、Tb、Dy的敏感性提高了5 ~ 20倍。通过静脉给药（25 mg元素/kg体重），测定Y或镧系元素在小鼠各器官中的浓度。给药20小时后，在脾、肺和肝中发现高浓度的给药元素。同样的器官分布也出现在给药10 mg/kg的Eu、Tb和Dy的小鼠身上。同时以10 mg/kg的剂量给药Eu、Tb和Dy，几乎等量的这些元素被纳入脾脏、肺和肝脏。脾脏、肺和肝脏钙浓度明显升高，且给药元素浓度较高。在所有器官中均未观察到稀土元素对Mg、Zn、Fe和P浓度的显著影响。给药元素的浓度和Ca浓度的增加比例（给药/对照）因器官和元素的不同而不同。然而，这些变化与稀土元素的化学或物理性质之间的关系尚不清楚。少

The rare earth elements are being used in the field of electronics, super conductors, alloys, nagnetic materials. and luminous materials. In order to investigate biological effects of rare earth elements distribution of these elements in organs of mice was observed after administration of rare earth compounds. The effects of the administration of these elements on the concentrations of biologically essential elements such as Ca. Mg. Zn. Fe. and P in organs was also observed.1. Quantitative methods for determination of yttrium(Y) and lanthanide elements in biological materials were developed. After dissolving organs of mice by simple wetwashing procedure, Eu. Tb, or Dy complex with 2.6-pyridinedicarboxylic acid was measured by fluorometry. The detection limit of each element in this method was ; I ng/mi in Eu, I ng/ml in Tb, or 5 ng/ml in Dy.2. All of the elements used (Y. La, Ce, Nd, Sm. Eu, Gd, Tb, Dy. Ho, Er, and Yb) were available to determine using ion chlomatography combined with … More post-column reaction using arsenazo-III solution. The detection limit of this method was 2.5 ng in each element. Sensitivities for Eu, Tb, and Dy were increased 5-20 fold by using 2, 6-pyridinedicarboxilic acid solution as post-column reaction reagent in comparison with arsenazo-III solution.3. The concentrations of Y or lanthanides in various organs of mice were determined after intravenous administration of their chloride compounds (25 mg element/kg body weight). Twenty hours after the administration, high concentrations of elements administered were found in spleen, lung, and liver. Similar organ distribution was found in mice administered 10 mg/kg Eu, Tb, of Dy. By the simultaneous administration of Eu, Tb, and Dy at 10 mg /kg each almost equql amounts of these elements were incorporated in spleen, as well as in lung and liver.4. The increase of Ca concentration was clealy observed in spleen, lung, and liver in which high concentrations of administered elements were found. No significant changes in Mg, Zn, Fe, and P concentrations by the administration with the rare earth elements were observed in all organs tested.5. The concentrations of administered elements and increase ratio of Ca concentration (administered/control) were different depending on the organ and element used. The relation between these changes and chemical or physical properties of rare earth elements, however, was not clear. Less