Immunologic and genetic analysis of diffuse panbronchiolitis

1.红霉素治疗弥漫性泛毛细支气管炎的免疫学分析：(1)红霉素对弥漫性泛毛细支气管炎患者外周血中活化T细胞的影响。提示EM抑制活化的T细胞。(2)EM对大鼠肺泡巨噬细胞产生LTB_4的影响。EM(100µg/ml)与巨噬细胞孵育6小时后，抑制大鼠肺泡巨噬细胞产生LTB_4。(3)罗红霉素对DPB患者的作用。罗红霉素是一种与EM具有相同化学结构的大环内酯类药物，对DPB有治疗作用。(4)EM对干燥综合征患者活化T细胞的影响。EM抑制干燥综合征患者外周血中活化T细胞的百分率。DPB患者家族中的人类白细胞抗原分析：分析了6个家族中的7个DPB患者和13个鼻窦炎患者(共32名成员)。13例鼻窦炎患者的HLA单倍型与DPB患者相同。这一结果有力地表明，易感基因(S)可能存在于人类白细胞抗原基因附近。

1. Immunologic analysis of effects of erythromycin(EM) on the patients with diffuse panbronchiolitis(DPB).(1) Effects of EM on the activated T cells in the patients with DPB.After EM therapy, percentages of activated CD3+, CD8+, and CD4+ cells in peripheral blood from DPS patients decreased in all patients. It was suggested that EM suppressed activated T cells.(2) Effect of EM on the production of LTB4 from rat alveolar macrophages.EM suppressed the production of LTB4 from rat alveolar macrophages, when macrophages were incubated with EM (100 mug/ml) for 6 hours.(3) Effect of roxithromycin on the patients with DPB.Roxithromycin, one of macrolides which have same chemical structure of EM, was curative of DPB.(4) Effect of EM on the activated T cells in the patients with Sjoegren's syndrome.EM suppressed percentages of activated T cells in peripheral blood of the patient with Sjoegren's syndrome.2. Analysis of HLA antigens in the family of DPB patients.Six families with 7 DPB patients and 13 sinusitis (total 32 members) were analyzed. Thirteen sinusitis patients had the same HLA haplotype of DPB patients. This results strongly suggested that susceptible gene(s) might exist near the HLA antigen genes.