STUDY ON MECHANISM OF EPIDERMAL EXCORIARION CAUSED BY EXFOLIATIVE TOXIN,USING MOUSE EMBRYOS

<材料与方法>取2日龄小鼠和6月龄小鼠（N2和N6m）的皮肤样本，以及胚胎第13 ~ 18天的小鼠胚胎（e13 ~ e18），光镜下观察阴离子位点。取N2、E15、E18皮肤样品进行电镜观察。用ET孵育E13-E18和N2的皮肤，我们观察到表皮内裂的定位。II . E13-E18、N2和N6m的皮肤分别经过以下预处理：a)未处理b)透明质酸酶c)肝素酶、软骨素酶ABC和角化酶，用聚乙烯亚胺（PEI）中和E15和N2表皮内的所有miii阴离子位点，并用精细阳离子钙酸铁胶体（Fe-cac-f）染色。fe -ca -f染色后，用电镜观察E15、E18和N2的皮肤。结果>IET诱导E14和E15表皮基底上裂形成。随着颗粒层的形成，解理面由基上层向颗粒层移动。在N2中，表皮内裂只发生在颗粒层内。在E13和E14的表皮相邻细胞之间发现了阴离子位点。在E15 ~ E18，阴离子位点在除基底层外的所有表皮层中都是废弃的。随着皮肤的成熟，阴离子位点集中在颗粒层。在E16-E18和N2的毛乳头细胞间隙中也有丰富的阴离子位点。透明质酸酶处理后，表皮II-b阴离子位点变化不大，但毛乳头阴离子位点明显减少。两个区域的II-c阴离子位点未被各自的酶处理改变。所有酶的处理对表皮没有影响，但减少了毛乳头的阴离子位点。III PEI灌注导致表皮内分裂，发生在E15表皮的基底上层和N2表皮的颗粒层内。我们观察到铁胶体颗粒在小鼠表皮各层任意细胞的细胞内和细胞间隙弥散分布。本研究未发现特异性阴离子位点。但在毛乳头的细胞间隙中发现了特定的阴离子位点。少

<Material and method>The skin samples of 2-day-old mice and 6-month-old mice (N2 and N6m), and mouse embryos on embryonic days 13 to 18 (E13-E18) were used for observation of anionic sites with light microscopy.The skin samples of N2, E15, and E18 were used for observation with electron microscopy.I Skin of E13-E18, and N2 were incubated with ET,and we observed localization of intraepidermal cleft.II Skin of E13-E18, N2, and N6m were stained with fine cationic cacodylate iron colloid (Fe-cac-f) after the following pre-treatments :a) non-treatment b) hyaluronidase c) heparitinase, chondroitinase ABC,and keratanase respectively, and all of themIII Anionic sites within the epidermis of E15 and N2 were neutralized with polyethyleneimine (PEI).IV Skin of E15, E18, and N2 were observed with EM after Fe-cac-f staining.<Results>IET induced suprabasal cleft formation in the epidermis of E14 and E15. As the granular layr formed, the cleavage plane shifted from the suprabasal to the granular layr … More . Finally intraepidermal clefts occured only within the granular layr in N2.II-a Anionic sites were found between adjacent cells in the epidermis of E13 and E14. On E15 to E18, anionic sites became abandant throughout all the layrs of the epidermis except the basal layr. As the skin matured, anionic sites concentrated in the granular layr. And anionic sites were also abundant in the intercellular spaces of hair papilla of E16-E18 and N2.II-b Anionic sites in the epidermis did not change so much, but those in the hair papilla were much decreased by hyaluronidase treatment.II-c Anionic sites of both regions were not altered by respective enzyme treatment. Treatment by all enzymes made no change in the epidermis, but decreased anionic sites of the hair papilla.III PEI perfusion resulted in intraepidermal splitting that occured within the suprabasal layr in the epidermis of E15, and within the granular layr in the epidermis of N2.IV We observed that Fe colloidal particles were distributed diffusely in intra- and intercellular spaces of any cells of all layrs in the epidermis of mice. Specific anionic sites were not found in this study. But specific anionic sites were found in intercellular spaces of hair pappila. Less