STUDY ON MECHANISM OF EPIDERMAL EXCORIARION CAUSED BY EXFOLIATIVE TOXIN,USING MOUSE EMBRYOS

小鼠胚胎剥脱性毒素引起表皮剥脱机制的研究

• 批准号: 07670949
• 负责人: ARATA Jiro
• 金额: $ 1.47万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670949/
• 关键词: EXFORIATIVE TOXIN; Fe-cac-f; ANIONIC SITES; HAIR PAPILLA; CHONDROITINASE ABC; HEPARRITINASE; KERATANASE; HYALURONIDASE; 表皮; 硫酸基; プロテオグリカン; グリコサミノグリカン; ヒアルロン酸; anionic site; cell-cell adhesion; development; embryonic skin; exfoliative toxi

<Material and method>The skin samples of 2-day-old mice and 6-month-old mice (N2 and N6m), and mouse embryos on embryonic days 13 to 18 (E13-E18) were used for observation of anionic sites with light microscopy.The skin samples of N2, E15, and E18 were used for observation with electron microscopy.I Skin of E13-E18, and N2 were incubated with ET,and we observed localization of intraepidermal cleft.II Skin of E13-E18, N2, and N6m were stained with fine cationic cacodylate iron colloid (Fe-cac-f) after the following pre-treatments :a) non-treatment b) hyaluronidase c) heparitinase, chondroitinase ABC,and keratanase respectively, and all of themIII Anionic sites within the epidermis of E15 and N2 were neutralized with polyethyleneimine (PEI).IV Skin of E15, E18, and N2 were observed with EM after Fe-cac-f staining.<Results>IET induced suprabasal cleft formation in the epidermis of E14 and E15. As the granular layr formed, the cleavage plane shifted from the suprabasal to the granular layr … More . Finally intraepidermal clefts occured only within the granular layr in N2.II-a Anionic sites were found between adjacent cells in the epidermis of E13 and E14. On E15 to E18, anionic sites became abandant throughout all the layrs of the epidermis except the basal layr. As the skin matured, anionic sites concentrated in the granular layr. And anionic sites were also abundant in the intercellular spaces of hair papilla of E16-E18 and N2.II-b Anionic sites in the epidermis did not change so much, but those in the hair papilla were much decreased by hyaluronidase treatment.II-c Anionic sites of both regions were not altered by respective enzyme treatment. Treatment by all enzymes made no change in the epidermis, but decreased anionic sites of the hair papilla.III PEI perfusion resulted in intraepidermal splitting that occured within the suprabasal layr in the epidermis of E15, and within the granular layr in the epidermis of N2.IV We observed that Fe colloidal particles were distributed diffusely in intra- and intercellular spaces of any cells of all layrs in the epidermis of mice. Specific anionic sites were not found in this study. But specific anionic sites were found in intercellular spaces of hair pappila. Less

<Material and method>2日龄和6月龄小鼠皮肤样品取胚胎13 ~ 18天（E13 ~ E18）的小鼠皮肤标本，用光镜观察阴离子位点，取胚胎13 ~ 18天（E13 ~ E18）的小鼠皮肤标本，用电镜观察阴离子位点。Ⅱ、E13-E18、N2、N6 m皮肤用细的阳离子二甲胂酸铁胶体染色（Fe-cac-f）在以下预处理之后：a）未处理B）透明质酸酶c）分别为肝素酶、软骨素酶ABC和角蛋白酶，并且所有这些都用聚乙烯亚胺（PEI）中和E15和N2的表皮内的阴离子位点。Fe-cac-f染色后电镜观察N2。<Results>IET诱导E14和E15的表皮形成基底上裂。随着颗粒层的形成，解理面由基底上向颗粒层移动， ...更多信息 .Ⅱ-a在E13和E14的表皮中，相邻细胞之间存在阴离子位点。E15 ~ E18时，除基底层外，表皮各层阴离子位点均丰富。随着皮肤的成熟，阴离子位点集中在颗粒层。E16-E18和N2毛乳头的细胞间隙也有丰富的阴离子位点。Ⅱ-b表皮的阴离子位点在透明质酸酶处理后变化不大，但毛乳头的阴离子位点明显减少。三种酶处理均未引起表皮的变化，但毛乳头的阴离子位点减少，Ⅲ PEI灌注导致表皮内分裂，E15表皮的基底上层和N2表皮的颗粒层内均发生分裂。Ⅳ我们观察到Fe胶体颗粒弥散分布于小鼠表皮各层任何细胞的细胞内和细胞间隙。本研究中未发现特异性阴离子位点。但在毛乳头的细胞间隙中发现了特异的阴离子位点。少

项目成果

E.Makino: "Are anionic sites involved in changes in cell layr suscetibility to exfoliative toxin during development?" Eur.J.Dermatol. vol6. 191-195 (1996)

E.Makino：“阴离子位点是否与发育过程中细胞层对剥脱性毒素敏感性的变化有关？”

Eiichi Makino: "Are Anionic Sites Involved in Changes in Cell Layer Susceptibility to Exfoliative Toxin Poring Epidermol Development?" European Journal of Dermatology. 6. (1996)

Eiichi Makino：“阴离子位点是否参与细胞层对剥脱性毒素渗透表皮摩尔发育敏感性的变化？”

E.Makino: "Are anionic sites involved in chages in cell layer susceptibility to exfoliative toxin during epidermal development?" European journal of dermatology. vol.6. 191-195 (1996)

E.Makino：“阴离子位点是否与表皮发育过程中细胞层对剥脱性毒素敏感性的变化有关？”