Biochemical Markers of Melanoma Utilizing Melanin Formation

利用黑色素形成的黑色素瘤的生化标志物

• 批准号: 02670487
• 负责人: ITO Shosuke
• 金额: $ 1.15万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670487/
• 关键词: Melanin; Malignant melanoma; Dopa; Normal values; HPLC; ド-パ

1. Significance of melanin-related metabolites as biochemical markers of melanoma(1) Normal values of 5-S-cysteinyldopa(5-S-CD) and 6-hydroxy-5-methoxyindole-2-carboxylic acid(6H5MI2C) in serum and urine were obtained from 33 healthy Japanese. The urinary excretion of both markers showed significant decrease in elderly subjects as compared with middle-aged subjects, while the serum concentration showed no age-dependent differences.(2) Values of those 4 markers in melanoma patients have regularly been followed. Among the 4 markers serum 5-S-CD appears to reflect most sensitively the progression of melanoma.Some patients showed pathological values of serum 5-S-CD(above 10 nmol/L) prior to the detection by image diagnosis. On the other hand, 6H5MI2C in serum and urine was found to be of little clinical significance.(3) Serum 5-S-CD values have been analyzed every month in 2 years' period in 10 healthy subjects. No values exceeded 10 nmol/L, the upper limit of normal value, in the 240 samples.2. Improvement of HPLC determination of serum 5-S-CD The previous method for the HPLC determination of serum 5-S-CD required 80 min for each chromatographic run. Pre-treatment of serum samples has been improved to reduce the time for each analysis to 40 min, which enabled to double the analytical capacity.

1. 黑色素相关代谢物作为黑色素瘤生化标志物的意义(1)测定了33例健康日本人血清和尿液中5- s -半胱氨酸多巴（5-S-CD）和6-羟基-5-甲氧基吲哚-2-羧酸（6H5MI2C）的正常值。老年受试者尿液中两种标志物的排泄量均明显低于中年受试者，而血清浓度无年龄依赖性。(2)定期跟踪黑色素瘤患者这4项指标的值。在4种标志物中，血清5-S-CD似乎最敏感地反映黑色素瘤的进展。部分患者在影像诊断检测前血清5-S-CD已达到病理值（10 nmol/L以上）。另一方面，血清和尿液中的6H5MI2C没有什么临床意义。(3)对10例健康受试者2年内每月血清5-S-CD值进行分析。在240份样本中，没有超过正常值上限10nmol /L的。高效液相色谱法测定血清5-S-CD的改进以往的高效液相色谱法测定血清5-S-CD每次运行需要80 min。改进了血清样品的预处理，将每次分析的时间减少到40分钟，使分析能力提高了一倍。

项目成果

Takashi HORILOSHI: "Serum 5-S-cysteinyldopa(5-S-CD)as a marker of melanoma progression" Journal of Dermatology. 19. 809-813 (1992)

Takashi HORILOSHI：“血清 5-S-半胱氨酰多巴 (5-S-CD) 作为黑色素瘤进展的标志物”皮肤病学杂志。

堀越 貴志,伊藤 祥輔,若松 一雄,小野寺 英夫,江口 弘晃,川村 邦子,高橋 誠: "血清5ーSーCD(5ーSーcysteinyldopa)の変動と悪性黒色腫の予後" 皮膚悪性腫瘍.

Takashi Horikoshi、Shosuke Ito、Kazuo Wakamatsu、Hideo Onodera、Hiroaki Eguchi、Kuniko Kawamura、Makoto Takahashi：“血清 5-SCD（5-S-半胱氨酰多巴）的变化和恶性黑色素瘤的预后”皮肤恶性肿瘤。

Kazumasa WAKAMATSU: "Comparison of melanin-related metabolites as markers of melanoma. Stability and correlation" Advances in Tryptophan Research 1992(Eds.I.Ishiguro,et al.),Fujita Health Univ.Press. 231-234 (1992)

Kazumasa WAKAMATSU：“黑色素相关代谢物作为黑色素瘤标志物的比较。稳定性和相关性”色氨酸研究进展 1992（Eds.I.Ishiguro 等人），Fujita Health Univ.Press。

Shosukee ITO: "Melanin-related metabolites as markers of melanoma:A review" Journal of Dermatology. 19. 802-805 (1992)

Shosukee ITO：“黑色素相关代谢物作为黑色素瘤的标志物：综述”皮肤病学杂志。

Kazumasa Wakamatsu;Shosuke Ito: "Urinary Excretion and Serum Concecentation of 5-S-Cysteinyldopa and 6-Hydroxy-5-methoxyindole-2-carboxylic Acid in Normal Subjects" Malanoma Research. 1. 141-147 (1991)

Kazumasa Wakamatsu；Shosuke Ito：“正常受试者中 5-S-半胱氨酰多巴和 6-羟基-5-甲氧基吲哚-2-羧酸的尿排泄和血清浓度”疟疾研究。