Elucidation of ultraviolet radiation-induced physiological degradation pathway of eumelanin and pheomelanin

阐明紫外线辐射诱导的真黑素和褐黑素的生理降解途径

• 批准号: 23591659
• 负责人: ITO Shosuke
• 金额: $ 3.33万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591659/
• 关键词: メラニン; 紫外線; 可視光線; ユーメラニン; フェオメラニン; UVA; UVB

Melanin is classified into black eumelanin and reddish pheomelanin. Our study aimed at elucidating UV-induced physiological degradation of both melanins. Using human black hair and red hair, we found that eumelanin is degraded to give PTCA (pyrrole-2,3,5-tricarboxylic acid) while pheomelanin is changed from benzothiazine to benzothiazole structure. Next, using mouse hair and human hair and synthetic eumelanin and pheomelanin, we found similar changes in structure of melanin upon UVA irradiation. Finally, using cultured melanocytes and retinal pigment epithelial cells, we showed that eumelanin gives rise to cross-linking structure upon UVA or visible light irradiation.

黑色素分为黑色真黑素和红色褐黑素。我们的研究旨在阐明紫外线诱导的两种黑色素的生理降解。以人的黑发和红发为研究对象，发现真黑素降解为PTCA（吡咯-2，3，5-三羧酸），而褐黑素则由苯并噻嗪结构转变为苯并噻唑结构。接下来，使用小鼠毛发和人类毛发以及合成的真黑素和褐黑素，我们发现UVA照射后黑色素的结构发生了类似的变化。最后，使用培养的黑素细胞和视网膜色素上皮细胞，我们表明，真黑素引起UVA或可见光照射后的交联结构。

项目成果

Oculocutaneous albinism 1 minimal pigment type : a case report on the analysis of genotype of an OCA1MP patient

眼皮肤白化病1最小色素型：OCA1MP患者基因型分析一例报告

• DOI: 10.1111/j.1365-2133.2011.10690.x
• 发表时间: 2012
• 期刊: Br.J.Dermatol.
• 作者: Kono;M.;Kondo;T.;Ito;S.;Suzuki;T.;Wakamatsu;K;et al
• 通讯作者: et al

Neutral pH and copper ions promote eumelanogenesis after the dopachrome stage

• DOI: 10.1111/pcmr.12137
• 发表时间: 2013-11-01
• 期刊: PIGMENT CELL & MELANOMA RESEARCH
• 影响因子: 4.3
• 作者: Ito, Shosuke;Suzuki, Natsumi;Wakamatsu, Kazumasa
• 通讯作者: Wakamatsu, Kazumasa

High-performance liquid chromatography estimation of cross-linking of dihydroxyindole moiety in eumelanin

• DOI: 10.1016/j.ab.2012.12.005
• 发表时间: 2013-03-15
• 期刊: ANALYTICAL BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Ito, Shosuke;Wakamatsu, Kazumasa;Simon, John D.
• 通讯作者: Simon, John D.

Evaluation of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin

碱性过氧化氢氧化分析真黑素和褐黑素的评价

• 发表时间: 2011
• 作者: Ito;S.;Nakanishi;Y.;Wakamatsu;K
• 通讯作者: K

Diversity of human hair pigmentation as studied by chemical analysis of eumelanin and pheomelanin

• DOI: 10.1111/j.1468-3083.2011.04278.x
• 发表时间: 2011-12-01
• 期刊: JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
• 影响因子: 9.2
• 作者: Ito, S.;Wakamatsu, K.
• 通讯作者: Wakamatsu, K.