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Regulation mechanism of melanin production by related-genes and UV.

相关基因和紫外线对黑色素生成的调控机制。

基本信息

批准号：
14570829
负责人：
ITO Shosuke
金额：
$ 1.92万
依托单位：
Fujita Health University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Contents of pheomelanin and its precursor, 5-S-cysteinyldopa in culture media from agouti (C57BL/10JHir-A/A) melanocytes was increased by L-tyrosine compared with black (a/a ; CS7BL/10JHir) melanocytes. Moreover, pheomelanin content in the epidermis from 3.5-and 5.5-day-old A/A mice was much higher than a/a mice. The A gene was expressed in the A/A dermis of 0.5-, 3.5-and 5.5-day-old mice, but not in the a/a dermis as well as in the A/A and a/a epidermis. These results suggest that pheomelanin production in the epidermis from 3.5-and 5.5-day-old A/A mice is induced by the expression of the agouti gene in the dermis.The study using melanocyte culture system of newborn mice wild-type at the pink-eyed dilution (p) locus (black ; C57BL/10JHir) and from congenic mice mutant at that locus (C57BL/10JHir-p/p) showed that the proliferation of p/p melanoblasts/melanocytes was stimulated by L-tyrosine (Tyr), and that the differentiation of melanocytes was induced by L-Tyr as the age of the donor mice advanced, even though eumelanin and pheomelanin failed to accumulate in p/p melanocytes and were released from them at all ages of skin development.Moreover, the study using melanocyte culture system of newborn mice wild-type at the slaty (sit) locus (black ; C57BL/10THir) and from congenic mice mutant at that locus (C57BL/10JHir-slt/slt) or of newborn mice wild-type at extension (e) locus (black : C57BL/10JHir) and from congenic mice mutant at that locus (C57BL/10JHir-e/e) showed eumelanin synthesis was inhibited in slt/slt melanocytes, and that both eumelanin and pheomelanin were synthesized in epidermis and dermis and their synthesis increased in e/e epidermis and dermis as skin development proceeded.

L-酪氨酸能增加黑素细胞培养液中的褐黑素及其前体5-S-半胱氨酰多巴的含量。此外，3.5和5.5日龄A/A小鼠表皮中的褐黑素含量远高于a/a小鼠。A基因在0.5、3.5和5.5日龄小鼠的A/A真皮中表达，但在a/a真皮以及A/A和a/a表皮中不表达。这些结果表明，3.5和5.5日龄A/A小鼠表皮中的褐黑素产生是由真皮中agglutinase基因的表达诱导的。（黑色; C57 BL/10 JHir）和来自该位点突变的同类小鼠（C57 BL/10 JHir-p/p）显示p/p成黑素细胞/黑素细胞的增殖被L-酪氨酸（Tyr）刺激，并且随着供体小鼠年龄的增长，黑素细胞的分化被L-Tyr诱导，即使真黑素和褐黑素不能在p/p黑素细胞中积累并且在皮肤发育的所有年龄从它们释放。使用新生小鼠野生型黑素细胞培养系统在Slaty（sit）位点的研究（黑色; C57 BL/10 THir）和来自该基因座突变的同类小鼠（C57 BL/10 JHir-slt/slt）或新生小鼠在延伸（e）基因座处的野生型（黑色：C57 BL/10 JHir）和来自该位点突变的同类小鼠（C57 BL/10 JHir-e/e）显示在slt/slt黑素细胞中真黑素合成受到抑制，真黑素和褐黑素均在表皮和真皮中合成，且随着皮肤发育，它们在E/E表皮和真皮中的合成增加。