Functions of Melanogenesis-Related Genes by Chemical and Biochemical Methods

通过化学和生化方法研究黑素生成相关基因的功能

基本信息

批准号：
07670971
负责人：
ITO Shosuke
金额：
$ 1.41万
依托单位：
Fujita Health University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670971/
关键词：
Eumelanin Pheomelanin Melanogenesis HPLC Spectrophotometric Method Hair Wool メラニンメラノサイトチロシナーゼ

项目摘要

Mammalian melanins exist in two chemically distinctforms : the brown to black eumelanins and the yellow to reddish pheomelanins. Melanogenesis is influenced by a number of genes, the levels of whose products determine the quantity and quality of the melanins produced. To examine the effects of various coat-color genes on the chemical properties of melanins synthesized in the follicular melanocytes of mice, we have introduced new methods to solubilize differentially pheomelanins and brown -type eumelanins. We applied these and previously developed HPLC and spectrophotometric methods for assaying eu- and pheomelanins to characterize melanins in various mutant mice : black, lethal yellow, viable yellow, agouti, brown, light, albino, dilute, recessive yellow, pink-eyed dilution, slaty, and silver. It was demonstrated that 1) complete solubilization of melanins in Soluene-350 is a convenient method to estimate the total amount of eu- and pheomelanins, 2) lethal yellow, viable yellow, and recessive yellow hairs contain almost pure pheomelanins, and 3) melanins from brown, light, silver, and pink-eyed black hairs share chemical properties in common that are characterized by partial solubility in strong alkali. We suggest that 1) the brown-type eumelanins have lower degrees of polymerization than the black-type eumelanins, and 2) slaty hair melanin contains a greatly reduced ratio of 5,6-dihydroxyindole-2-carboxylic acid-derived units as compared with black and other eumelanic hair melanins. These results indicate that our methodology, HPLC and spectrophotometric methods combined, may be useful in chemically characterizing melanin pigments produced follicular melanocytes.

哺乳动物黑色素存在于两个化学上不同的形式中：棕色至黑色的依美酸酯和黄色至红色的芬太尼。黑色素发生受许多基因的影响，其产物的水平决定了产生的黑色素的数量和质量。为了检验各种涂层基因对小鼠卵泡黑素细胞合成的黑色素的化学特性的影响，我们引入了新方法，以溶解差异化的芬氏素和棕色 - 型Eumelanins。我们应用了这些且先前开发的HPLC和分光光度法，用于测定Eu-和pheomelanins在各种突变小鼠中表征黑色素：黑色，致命黄色，可行的黄色，Agouti，Agouti，Agouti，Agouti，Aguti，Brown，Light，light，birt，稀释，稀释，隐性黄色，粉红色，粉红色的稀释，淡淡的稀释性，slaty和银色。 It was demonstrated that 1) complete solubilization of melanins in Soluene-350 is a convenient method to estimate the total amount of eu- and pheomelanins, 2) lethal yellow, viable yellow, and recessive yellow hairs contain almost pure pheomelanins, and 3) melanins from brown, light, silver, and pink-eyed black hairs share chemical properties in common that are characterized by partial solubility in strong alkali.我们建议1）与黑型eumelanins相比，棕色的eumelanin具有低度的聚合度，而2）与黑色的黑色素黑色素相比，黑色素黑色素的比例大大降低了5,6-二羟基2-羧酸含有5,6-二羟基 - 二羧酸的单元，与黑人和其他黑色和其他黑色和其他黑色的头发相比。这些结果表明，我们的方法，HPLC和分光光度计方法合并，可能可用于化学表征黑色素色素产生的卵泡黑色素细胞。