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Basic study of Cultured Artificial Skin for Future Clinical Application and Its assesment for invitro Experimental Model

未来临床应用的培养人造皮肤的基础研究及其体外实验模型的评估

基本信息

批准号：
05454352
负责人：
SUZUKI Shigehiko
金额：
$ 2.18万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454352/
关键词：
Skin Equivalent Artificial Skin Wound Dressing Cultured Epithelium Collagen Fibroblast Wound Healing Experimental Model 分化

项目摘要

1) A new cellular artificial skin composed of an outer keratinocytic layr and an inner collagen sponge containing fibroblasts was produced and its morphology was studied ultrastructurally and immunohistochemically. The histological appearance of keratinocytic layr was similar to that of normal skin. The production of fibronectin was confirmed with immunohistochemical staining. The results of immunohistochemical staining of the keratinocytic layr in the cellular artificial skin using some kinds of anti-keratin antibodies showed almost normal differentiation.The structure of the basement membrane zone in the cellular artificial skin also resembled that of normal skin ultrastructurally.2) The cellular artificial skin, the collagen sponge containing only fibroblasts and the acellular collagen sponge were grafted onto the skin defect produced in the back of nude mouse. The defect where the cellular artificial skin was grafted was epithelialized earliest. The defect used the collagen sponge containing fibroblasts was secondly epithelialized.3) An acellular "bilayr artificial skin" was successfully used clinically. The newly synthesized tissue by the artificial skin after long following-up indicates more similarity to the normal dermis than the scar tissue light microscopically and electromicroscopically.

1)本文报道了一种由外层角质形成细胞和内层胶原海绵组成的新型细胞人工皮肤，并对其进行了超微结构和组织化学研究。角质形成细胞层的组织学形态与正常皮肤相似。免疫组化染色证实纤维连接蛋白的产生。用多种抗角蛋白抗体对细胞人工皮肤的角蛋白细胞层进行免疫组化染色，结果显示细胞人工皮肤的角蛋白细胞层分化基本正常，基底膜区的结构与正常皮肤的超微结构相似。将仅含成纤维细胞的胶原海绵和脱细胞胶原海绵移植到裸鼠背部产生的皮肤缺损上。细胞人工皮肤移植的缺损处上皮化最早。用含成纤维细胞的胶原海绵对缺损部位进行二次上皮化。3）脱细胞双层人工皮肤成功应用于临床。人工皮肤经长期追踪后，其新生组织在光镜及电镜下较疤痕组织更接近正常真皮。