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Local Factors in Bone Metabolism of Experimental Osteoporosis

实验性骨质疏松症骨代谢的局部因素

基本信息

批准号：
02670654
负责人：
OKUMURA Hideo
金额：
$ 1.54万
依托单位：
Ehime University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670654/
关键词：
Osteoporosis Rat Northern blot analysis m-RNA Local factor Osteocalcin 骨芽細胞活性型ビタミンD_3 実験的骨粗鬆症ノ-ザンブロッティング法 Cーfos cーDNA mーRNA TGFーβ CーDNA

项目摘要

Human osteoporosis is induced by aging, menopause and immobilization. Various hormones which regulate calcium metabolism in the whole body and various local factors which regulate bone metabolism in local bony tissue affect on the pathophysiology of osteoporosis. The expression of mRNA of local factors in the bone cells of osteoporotic tissue induced by immobilization combined with ovariectomy was studied. The mRNA was analyzed by Northern blot method using cDNA probes. The osteocalcin mRNA level in the osteoporotic tibiae significantly increased. The TGF-beta mRNA level increased in bone marrow cells and slightly increased in bone cells in the osteoporotic tibiae. The experiment of administration of dexamethasone was also done. The osteocalcin mRNA level in the tibiae of the rats treated with dexamethasone significantly decreased. Suppressive effect of dexamethasone on osteocalcin expression was persistent for seven days at higher dose. Northern blot analysis showed that both 1,25(OH)_2D_3 and 2beta-(3-hydroxypropoxy)-1,25(OH)_2D_3 caused an increase in osteocalcin mRNA levels in bone. And they increased osteocalcin mRNA levels in osteoblast-like cells ( ROS17/2.8 ) in vitro. In conclusion, general factors ( estrogen, 1,25(OH)_2D_3 ) have an effect on the trascriptional regulation of local factors ( osteocalcin, TGF- beta ) in bone.

人类骨质疏松症是由衰老、绝经和制动引起的。调节全身钙代谢的各种激素和调节局部骨组织骨代谢的各种局部因素影响骨质疏松症的病理生理。本文研究了制动联合卵巢切除对骨质疏松组织骨细胞局部因子mRNA表达的影响。用cDNA探针进行北方印迹分析。骨钙素mRNA水平在骨质疏松的胫骨中显著升高。TGF-β mRNA水平在骨髓细胞中增加，在骨细胞中略有增加。并进行了地塞米松给药实验。地塞米松治疗组大鼠胫骨骨钙素mRNA水平显著降低。地塞米松对骨钙素表达的抑制作用在较高剂量下持续7天。北方印迹分析表明，1，25（OH）_2D_3和2 β-（3-羟基丙氧基）-1，25（OH）_2D_3均能引起骨钙素mRNA水平的升高。体外培养的成骨样细胞（ROS 17/2.8）中骨钙素mRNA水平增加。结论：全身因素（雌激素、1，25（OH）_2D_3）对骨局部因素（骨钙素、TGF-β）的转录调节有影响。