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Electrophysiological and morphological study of endolymphatic sac

内淋巴囊的电生理和形态学研究

基本信息

批准号：
02670766
负责人：
MORI Nozomu
金额：
$ 1.54万
依托单位：
Kagawa Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670766/
关键词：
endolymphatic sac direct current potential epithelial cell catecholamine acetazolamide macromolecule

项目摘要

(Electrophysiological Study)The endolymphatic sac direct current potential(ESP)was measured in the guinea pig by means of a glass microelectrode, and the effect of anoxia and various agents on the ESP was examined to know its properties. The results are as follows : 1)The ESP is composed of an anoxia-sensitive part and an anoxiainsensitive part. 2)Canrenoate, an aldosterone antagonist, decreased the ESP in a dose-dependent manner. 3)Acetazolamide, a carbonic anhydrase inhibitor, produced a dose-dependent decrease in the ESP with a saturation of its effect at large doses. 4)Catecholamines reversibly decreased the ESP by beta-adrenergic action. 5)The anoxia-sensitive part of the ESP consists of catecholamine-sensitive and acetazolamide-spnsitve parts.(Morphological Study)Ultrastructure of the endolymphatic sac(ES)in the mouse and the transport of macromolecule in the ES were observed. The results are as follows : 1)The ES in the mouse was divided into three parts : proximal, intermediate and distal. 2)The epithelial cells in the intermediate portion were classified into two types : type I and type II. Type I cells had abundant microvilli, pinocytotic vesicles, vacuoles, multivesicular bodies, lysosomes and mitochondria. Type II cells had fewer numbers of these organelles. 3)The capillaries of the ES were classified as either fenestrated or non-fenestrated. They were botn considered to be permeable to macromolecules. The basolateral absorption in the ES epithelium was considered to be active especially in the intermediate portion.

（电生理学研究）用玻璃微电极测定豚鼠内淋巴囊直流电位（ESP），并观察缺氧及各种药物对ESP的影响，以了解ESP的性质。结果表明：1）ESP由缺氧敏感区和缺氧不敏感区组成。（2）醛固酮拮抗剂坎利酸酯呈剂量依赖性地降低ESP。3）乙酰唑胺是一种碳酸酐酶抑制剂，其作用呈剂量依赖性，在大剂量时达到饱和。4)儿茶酚胺通过β-肾上腺素能作用可逆地降低ESP。5)ESP的缺氧敏感部分由儿茶酚胺敏感部分和乙酰唑胺敏感部分组成。形态学观察了小鼠内淋巴囊（ES）的超微结构及ES内大分子物质的转运。结果如下：1）小鼠ES分为近端、中间端和远端三部分。2)中间部的上皮细胞分为两种类型：I型和II型。I型细胞具有丰富的微绒毛、胞饮小泡、空泡、多泡体、溶酶体和线粒体。II型细胞的这些细胞器数量较少。3)ES的毛细血管分为有孔或无孔。它们被认为是可渗透的大分子。ES上皮的基底侧吸收被认为是活跃的，尤其是在中间部分。