Research on the role of βadrenergic receptors in pathogenesis of Meniere's disease

β肾上腺素能受体在梅尼埃病发病机制中的作用研究

• 批准号: 22591883
• 负责人: MORI Nozomu
• 金额: $ 2.83万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591883/
• 关键词: メニエール病; β受容体; 内リンパ嚢; 内リンパ圧; ストレス; ラット内リンパ嚢; 上皮細胞; 中間部; アドレナリン受容体; RT-PCR; 免疫組織学; β_2-AR; β_3-AR; βアドレナリン受容体; カテコールアミン; βアゴニスト; 内耳圧

The results that catecholamines increased endolymphatic hydropressure in the cochlea and semicircular canalin the normal guinea pigs but not in the guinea pigs with obstructed endolymphatic sac (ES) strongly suggest the involvement of ES in increased endolymphatic hydropressure by catecholamines. The findings that catecholamines increased endolymphatic hydropressure in the cochlea to a larger degree than that in the semicircular canalcorrespond to clinical findings that Meniere patients suffer more frequently from the cochlear symptoms than the vestibular symptoms. The strong expression of β2-adrenergic receptor in epithelial cells of the ES intermediate portion accords with the electrophysiological results that catecholamines decrease ES direct current potentialvia β2-action.

儿茶酚胺可使正常豚鼠耳蜗和半规管内淋巴水压升高，但对内淋巴囊阻塞的豚鼠无明显影响，提示儿茶酚胺参与了儿茶酚胺升高内淋巴液压力的作用。儿茶酚胺对耳蜗内淋巴水压的增加幅度大于半规管，这与梅尼埃患者耳蜗性症状多于前庭症状的临床发现相一致。ES中间部上皮细胞β_2-肾上腺素能受体的强阳性表达与儿茶酚胺通过β_2作用降低ES直流电的电生理结果一致。

项目成果

Laser Capture Microdissection : Methods and Protocols, Methods in Molecular Biology, Vol.755

激光捕获显微切割：方法和方案，分子生物学方法，第 755 卷

• 发表时间: 2011
• 作者: Akiyama;K.;et al
• 通讯作者: et al

Evaluation of the vestibular aqueduct in Meniere's disease using multiplanar reconstruction images of CT

• DOI: 10.1016/j.anl.2011.11.005
• 发表时间: 2012-12-01
• 期刊: AURIS NASUS LARYNX
• 影响因子: 1.7
• 作者: Miyashita, Takenori;Toyama, Yoshihiro;Mori, Nozomu
• 通讯作者: Mori, Nozomu

The mRNA of claudins is expressed in the endolymphatic sac epithelia.

• DOI: 10.1016/j.anl.2011.07.004
• 发表时间: 2012-08
• 期刊: Auris, nasus, larynx
• 作者: Ai Matsubara;T. Miyashita;Terushige Mori;Kosuke Akiyama;Ryuhei Inamoto;Nozomu Mori

イソプロテレノールの内耳への影響

异丙肾上腺素对内耳的影响

• 作者: Fuchiwaki T;Sun X;Fujimura K;Yamada H;Shibata K;Muta H;Podack ER;Kawauchi H;Yoshikai Y;淵脇貴史;淵脇貴史;淵脇貴史;淵脇貴史;稲本 隆平;稲本 隆平;稲本 隆平
• 通讯作者: 稲本 隆平

Presence of adrenergic receptors in the rat endolymphatic sac epithelial cells.

大鼠内淋巴囊上皮细胞中存在肾上腺素能受体。

• 发表时间: 2012
• 作者: Matsubara,A.;T. Miyashita;T..Inamoto and N. Mori
• 通讯作者: T..Inamoto and N. Mori