Electrophysiological and morphological study of endolymphatic sac

内淋巴囊的电生理和形态学研究

• 批准号: 05454462
• 负责人: MORI Nozomu
• 金额: $ 4.03万
• 依托单位: Kagawa Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454462/
• 关键词: endolymphatic sac; epithelial cell; patch clamp technique; outward potassium current; macromolecule transport; immunohistochemistry; carbonic anhydrase; 内リンパ嚢; 直流電位

(Electrophysiological Study) The whole cell currents were measured in freshly isolated epithelial cells from the intermediate portion of the endolymphatic sac (ES) in the guinea pig by patch clamp technique. In most cells the outwardly delayd rectifying current was evoked by depolarizing voltage pulses. The reversal potential of the current was close to the potassium equilibrium potential, indicating that the outward current is carried mostly by potassium. The results suggest that the delayd rectifying current may play an important role in the potassium ion transport in the epithelial cells of the ES.(Morphological Study) 1) To investigate the detailed absorption activity and the barrier properties of the ES,a constant volume of horseradish peroxidase (HRP) aws injected directly into the ES lumen of the guinea pig. The epithelial cells in the proximal portion did not absorb the intraluminal HRP at any intervals after the tracer injection. The epithelial cells of the intermediate portion were classified clearly into two types according to their sbsortion activity : active-abosorptive and non-active cells. The absorption activity in the distal portion was lower than that in the active-absorptive cells in the intermediate portion. The epithelial linings of the ES showed a tight barrier to HRP.2) All epithelial cells in the distal portion and about a half the epithelial cells of the intermediate portion stained for carbonic anhydrase (CA). The proximal portion of the ES lacked immunoreactivity. The results suggest that CA may play a crucial role inthe electrolyte transport of the ES.

（电生理学研究）用膜片钳技术测定了豚鼠内淋巴囊（ES）中部新鲜分离的上皮细胞的全细胞电流。在大多数细胞中，去极化电压脉冲可诱发外向延迟整流电流。电流的反向电位接近钾平衡电位，表明外向电流主要由钾携带。结果提示延迟整流电流在ES上皮细胞钾离子转运中起重要作用。（形态学研究）1）将一定体积的辣根过氧化物酶（HRP）直接注射到豚鼠ES腔中，研究ES的详细吸收活性和屏障特性。近端部分的上皮细胞在示踪剂注射后的任何时间间隔都不吸收管腔内HRP。中间部上皮细胞按其吸收活性可分为吸收活性细胞和非吸收活性细胞。远侧部的吸收活性低于中间部的活性吸收细胞。ES的上皮衬里显示出对HRP的紧密屏障。2）远端部分的所有上皮细胞和中间部分的约一半上皮细胞进行碳酸酐酶（CA）染色。ES的近端部分缺乏免疫反应性。结果提示CA可能在ES的电解质转运中起重要作用。

项目成果

Hoshikawa, H., Furuta, H., Mori, N.and Sakai, S.: "Morphometric analysis of macromolecular absorption and barrier properties in each portion of the endolymphatic sac." Proceeding Sendai Symposium. 3. 37-42 (1993)

Hoshikawa, H.、Furuta, H.、Mori, N. 和 Sakai, S.：“内淋巴囊各部分大分子吸收和屏障特性的形态计量分析”。

H.Hoshikawa: "Morphometic dadlysis of macro molecular absorpfron and barner properties in each protion of the end Olyaphatic sdc" Proc.Sendai Symposium. 3. 37-42 (1993)

H.Hoshikawa：“Olyaphatic sdc 末端各部分中大分子吸收和 Barner 特性的形态学分析”Proc.Sendai Symposium。

Furuta, H., Mori, N.and Sakai, S.: "Immunohistochemical localization of carbonic anhydrase in the endolymphatic sac of the mouse and guinea pig." ORL. 55. 13-17 (1993)

Furuta, H.、Mori, N. 和 Sakai, S.：“小鼠和豚鼠内淋巴囊中碳酸酐酶的免疫组织化学定位。”

Mori, N., Sakagami, M., Fukazawa, K.and Matsunaga, T.: "Immunohistochemical and electrophysiological study in Isk protein in the stria vascularis of guinea pig." Eur. Arch. Otorhinolaryngol. 250. 186-189 (1993)

Mori, N.、Sakagami, M.、Fukazawa, K. 和 Matsunaga, T.：“豚鼠血管纹中 Isk 蛋白的免疫组织化学和电生理学研究。”

N.Mori: "An immuno histo chemical and electrophysrolgrcal study on Lsk protein in the styad vasculions of the gumedpiy" Eur.Arch,Otorhino la ryngol. 250. 186-189 (1993)

N.Mori：“对胶质血管中 Lsk 蛋白的免疫组织化学和电生理研究”Eur.Arch，Otorhino la ryngol。