Core B - Biomolecular tools
核心 B - 生物分子工具
基本信息
- 批准号:10628927
- 负责人:
- 金额:$ 37万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-07-01 至 2028-04-30
- 项目状态:未结题
- 来源:
- 关键词:Age related macular degenerationAgingAlkaline PhosphataseAlzheimer&aposs DiseaseAmyloid beta-ProteinBiochemicalBiologicalBiological AssayBiologyBiophysicsCellsChemicalsChemistryCysteineData AnalysesDevelopmentDiphosphatesDiseaseDrusenEscherichia coliEtiologyEventEyeFluorescence SpectroscopyGoalsHealthHydroxyapatitesImageIn VitroIsotope LabelingLabelLengthLipidsMammalian CellMethodsModificationMolecularMusNuclear Magnetic ResonanceNuclear ProteinsParticipantPatientsPhosphorylationPost-Translational Protein ProcessingPreparationProcessProductionPropertyProteinsProtocols documentationReagentRoleSamplingStructural BiologistStructure of retinal pigment epitheliumTestingTimeTissuesValidationVitronectinWorkcalcificationdata structuredesignexperimental studyimaging studyinorganic phosphateinteinnovelparticlesensorstructural biologytau Proteinstool
项目摘要
CORE B SUMMARY
Protein Production and Chemical Biology. The overall goal of this P01 proposal is to elucidate the biological
mechanisms of calcification and their potential role in the onset and etiology of age-related macular
degeneration (AMD) and Alzheimer’s disease (AD). The proposed projects will investigate calcification at the
molecular, cellular and organismal levels to build a comprehensive view of the participants that are responsible
for this aging-related phenomenon. Central to all these efforts will be the availability of pure and homogenous
protein reagents with defined properties and modifications that can be manipulated in the various assays and
experiments proposed in the projects. The goal of this Core will be to design and prepare these reagents, with
a particular focus on the calcification-associated proteins vitronectin (Vn), Tau and amyloid β (Aβ). The Core
will also aim to develop enhanced tools for the production of modified proteins for structural and imaging
studies. More specifically, the Core will work towards the following goals: 1) Production of Vn, Tau, and Aβ
constructs for structural, biochemical, biophysical and cellular analysis as necessary for all projects in the
proposal; 2) Development of intein-based tools for segmental isotopic labeling of proteins for nuclear magnetic
resonance (NMR) studies as described in Project 1; and 3) Preparation of fluorescently labeled proteins for
biophysical assays and sensor development for all projects. In addition to serving the projects described in this
proposal, we expect that the protocols and reagents developed by the Core will be valuable tools for chemical
and cell biologists, biophysicists and structural biologists studying a wide range of biological problems in health
and disease.
核心B摘要
蛋白质生产和化学生物学。本P01提案的总体目标是阐明
钙化机制及其在老年性黄斑病变发病和病因学中的潜在作用
退行性变(AMD)和阿尔茨海默病(AD)。拟议的项目将调查钙化在
分子、细胞和有机体水平,以全面了解负责的参与者
对于这种与衰老相关的现象。所有这些努力的核心将是获得纯的和同质的
具有确定的性质和修饰的蛋白质试剂,其可以在各种测定中操作,
项目中提出的实验。本核心的目标是设计和制备这些试剂,
特别关注钙化相关蛋白玻连蛋白(Vn)、Tau和淀粉样蛋白β(Aβ)。核心
还将致力于开发用于生产结构和成像用改性蛋白质的增强工具
问题研究更具体地说,核心将朝着以下目标努力:1)生产Vn、Tau和Aβ
结构,生物化学,生物物理和细胞分析所需的所有项目的建设,
2)开发基于内含肽的工具,用于核磁共振的蛋白质片段同位素标记
如项目1中所述的核磁共振(NMR)研究;和3)制备荧光标记的蛋白质,
为所有项目提供生物物理分析和传感器开发。除了服务于本报告所述的项目外,
我们希望核心开发的方案和试剂将成为化学分析的宝贵工具。
细胞生物学家、生物药理学家和结构生物学家研究健康方面的各种生物学问题
和疾病
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Francesca M Marassi其他文献
Francesca M Marassi的其他文献
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{{ truncateString('Francesca M Marassi', 18)}}的其他基金
Molecular mechanisms of calcification: roles and opportunities in diseases of aging
钙化的分子机制:在衰老疾病中的作用和机会
- 批准号:
10628925 - 财政年份:2023
- 资助金额:
$ 37万 - 项目类别:
Membrane Protein Effectors of Pathogen Interactions With Host
病原体与宿主相互作用的膜蛋白效应子
- 批准号:
10630318 - 财政年份:2016
- 资助金额:
$ 37万 - 项目类别:
Membrane Protein Effectors of Pathogen Interactions With Host
病原体与宿主相互作用的膜蛋白效应子
- 批准号:
10207010 - 财政年份:2016
- 资助金额:
$ 37万 - 项目类别:
Membrane protein effectors of pathogen interactions with host
病原体与宿主相互作用的膜蛋白效应子
- 批准号:
9071833 - 财政年份:2016
- 资助金额:
$ 37万 - 项目类别:
Membrane Protein Effectors of Pathogen Interactions With Host
病原体与宿主相互作用的膜蛋白效应子
- 批准号:
10689583 - 财政年份:2016
- 资助金额:
$ 37万 - 项目类别:
Membrane protein effectors of pathogen interactions with host
病原体与宿主相互作用的膜蛋白效应子
- 批准号:
9276714 - 财政年份:2016
- 资助金额:
$ 37万 - 项目类别:
Membrane Protein Effectors of Pathogen Interactions With Host
病原体与宿主相互作用的膜蛋白效应子
- 批准号:
10404547 - 财政年份:2016
- 资助金额:
$ 37万 - 项目类别:
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