Study on muscle fiber composition and biochemical properties of the adductor mandibulae in fishes showing different feeding activities

不同摄食性鱼类下颌内收肌肌纤维组成及生化特性研究

• 批准号: 02670813
• 负责人: MAEDA Norihiko
• 金额: $ 1.41万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670813/
• 关键词: Fish; Adductor mandibulae; Muscle fiber composition; succinic dehydrogenase; LDH isozymes; フナ; ウグイ; オイカワ; 閉顎筋; 筋線維構成; LDHアイソザイム; Oncorhynchus masou; 開顎筋; Lactate dehydrogenase(LDH)活性; アイソザイムパタ-ン

Lactate dehydrogenase (LDH) isozyme distributions in the jaw-closing and -opening muscles, myotome muscle, heart, and eye of the parr-type and smolt-type fish in a primitive teleostean, Oncorhynchus masou (O.masou),which have adapted to the rapid current at the upper reach of a river and in the mild current at the middle reach,respectively, were investigated by horizontal agarose gel electrophoresis. Both jaw muscles of the parr-type fish had following three isozymes: the slowest anodal migrative LDHA_4, the fastest anodal migrative LDHB_4, and an isozyme migrating between them. The major isozyme was LDHA_4, amounting over 80% of total LDH activity. The white portion of the myotome muscle which is the dominant part and contracts fast and forcefully, and the heart muscle which contracts slowly and continuously had only one isozyme LDHA_4 or LDHB_4, respectively. The red portion of the myotome muscle had three isozymes, though LDHB_4 one was predominant. These facts suggest that jaw-clos … More ing and-opening muscles and myotome muscle are well adapted for swift feeding behavior in the rapid current, because forceful and rapid contractions of those muscles would be needed to catch the insects floating down in the rapid current. With growth of length of the fish, LDHB_4 isozyme activity significantly increased in the jaw-closing muscle, but not in the jaw-opening muscle. Furthermore, the smolt-type fish which lives in open-habitats of rivers and lakes and feed mainly on other species of fish, had a higher level of LDHB_4 isozyme in the jaw-closing muscle than the parr-type fish.Thus, the higher levels of LDHB_4 isozyme in the jaw-closing muscles of the larger parr-type fish and the smolt-type fish, and appearance of LDHB_4 isozyme in the white portion of the myotome muscle of the smolt-type fish, suggest that the function of the muscles may change with alternation of food habits and locomotion manners respectively, in this species. Moreover, these data may be very important for explanation of the physiological meaning of each type of isozyme of LDH in the muscles. Less

采用水平琼脂糖凝胶电泳技术，研究了分别适应河流上游急流和中游缓流条件的原始硬骨鱼虹鳟（Oncorhynchus masou，O.masou）幼鱼型和幼鱼型鱼类的颌张肌、肌节肌、心脏和眼睛中乳酸脱氢酶（LDH）同工酶的分布。Parr型鱼的两种颌肌都有三种同工酶：最慢的阳极迁移型LDHA_4、最快的阳极迁移型LDHB_4和一种在它们之间迁移的同工酶。主要同工酶为LDHA_4，占LDH总活性的80%以上。肌节肌的白色部分为优势部分，收缩快而有力;心肌的收缩慢而持续，分别只有一种同工酶LDHA_4或LDHB_4。肌节肌的红色部分有三种同工酶，但以LDHB_4同工酶为主。这些事实表明， ...更多信息 张开肌和肌节肌很好地适应了急流中的快速进食行为，因为需要这些肌肉的有力和快速收缩来捕捉急流中漂浮下来的昆虫。LDHB_4同工酶活性随体长的增长在闭颌肌中显著增加，而在开颌肌中无显著变化。此外，生活在江河湖泊开阔生境中，以其他鱼类为食的二龄鲑型鱼的闭颌肌中LDHB_4同工酶水平高于公鲑型鱼，因此，公鲑型鱼和二龄鲑型鱼的闭颌肌中LDHB_4同工酶水平较高，以及LDHB_4同工酶在二龄鲑肌节肌肉白色部分的出现，提示该种肌肉的功能可能分别随食性和运动方式的改变而改变。此外，这些数据可能是非常重要的解释各种类型的LDH同工酶在肌肉中的生理意义。少

项目成果

Mitsuru ICHIKAWA: "Distribution patterns of lactate dehydrogenase isozymes in the jaw and myotome muscles as influenced by habits of feeding and locomotion in the Salmonidae fish,Oncorhynchus masou and physiological meaning of the isozymes" Biomedical Res

Mitsuru ICHIKAWA：“乳酸脱氢酶同工酶在下颌和肌节肌肉中的分布模式受鲑鱼科鱼、虹鳟鱼摄食和运动习惯的影响以及同工酶的生理意义”生物医学研究

Mitsuru Ichikawa: "Distribution patterns of lactate dehydrogenase isozymes in the jaw and myotome muscles as influenced by habits of feeding and locomotion in the Salmonidae fish, Oncorhynchus masou and the physiological meaning of the isozymes." Biomedic

Mitsuru Ichikawa：“乳酸脱氢酶同工酶在下颌和肌节肌肉中的分布模式受鲑鱼科鱼类 Oncorhynchus masou 的摄食和运动习惯的影响，以及同工酶的生理意义。”