Basic study on the factors regulating the postnatal development of the masticatory organ and center

出生后咀嚼器官及中枢发育调控因素的基础研究

• 批准号: 05454489
• 负责人: MAEDA Norihiko
• 金额: $ 2.24万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454489/
• 关键词: Masticatory system; Masseter muscle; Postnatal development; Sexual dimorphism; Androgenic hormones; Peridontal ligaments; Trigeminal motor neuron; Trigeminal sensory neuron; Op; op mouse; Masseter muscle; Trigeminal motoneuron; Periodontal lig

Postnatal changes in the masticatory system and regulation factors were investigated, and following results were obtained.1.Sexual dimorphism of isozyme patterns of lactate dehydrogenase gradually appeared in the masseter muscle of developing mice. Testectomy inhibited the appearance, but not ovariectomy.2.Androgenic hormones induced the sexual differences in the masseter muscle. Cyproterone acetate which is an androgen antagonist inhibited the induction.3.Androgen receptors were certainly observed in masseter muscles of male and female mice. The receptors apppeared with maturation of male and female animals. No sexual difference was observed.4.Short-term denervation of the masseter nerve did not inhibit the appearance of sexual dimorphism in the masseter muscle.5.Sexual dimorphism in the trigeminal motor neurons innervating the mouse masseter muscle was observed. Estrogenic and androgenic hormones were involved in induction of the dimorphism.6.Less-developed periodontal ligaments in op/op mice caused the precocious atrophy of the masseter muscle, and induced the reduction in the number of motor and sensory neurons involved in the jaw-opening reflex in the mice.

结果表明：1.发育过程中小鼠咬肌中乳酸脱氢酶同工酶逐渐出现性别二型性。切除抑制了外观，但不影响卵巢切除。2.雄激素引起咬肌性别差异。雄激素拮抗剂醋酸环丙孕酮抑制了这种诱导。3.雄激素受体在雌雄小鼠咬肌中均可观察到。这些受体随着雌雄动物的成熟而出现。短期切断咬肌神经并不能抑制咬肌中性二型性的出现。5.在支配小鼠咬肌的三叉神经运动神经元中观察到两性二型性。6.OP/OP小鼠牙周韧带发育不良导致咬肌性早熟萎缩，并导致参与张口反射的运动神经元和感觉神经元数量减少。

项目成果

Suemune Setsuko: "Reduction of masticatory motoneurons in the trigeminal motor nucleus of the toothless (op/op) mouse." Biomedical Research. 15. 45-49 (1994)

Suemune Settsuko：“无牙（op/op）小鼠三叉神经运动核中咀嚼运动神经元的减少。”

Maeda, N., Kawata, T., Yoshiko, Y., Hosoi, M., Suemune, S., Okada, N.and Tanne, K.: "Postnatal changes in the masseter muscle of the toothless (op/op) mouse with less-developed periodontal ligaments" Biomedical Res.15. 255-261 (1994)

Maeda, N.、Kawata, T.、Yoshiko, Y.、Hosoi, M.、Suemune, S.、Okada, N. 和 Tanne, K.：“无牙者咬肌的产后变化（op/op）

Suemune, S.and Maeda, N.: "Reduction in the number of motor and sensory neurons that are involved in the jaw-opening reflex in the toothless (op/op) mouse" Biomedical Res.15. 263-269 (1994)

Suemune, S. 和 Maeda, N.：“减少无牙 (op/op) 小鼠张颌反射中涉及的运动和感觉神经元数量”Biomedical Res.15。

Maeda Norihiko: "Postnatal changes in the masseter muscle of the toothless (op/op) mouse with less‐developed periodontal ligaments." Biomedical Research. 15. 255-261 (1994)

Maeda Norihiko：“牙周韧带发育不良的无牙 (op/op) 小鼠咬肌的产后变化。” 15. 255-261 (1994)。

Maeda Norihiko: "Effects of denervation on the expression of lactate dehydrogenase isozymes in the masseter muscle of developing mice." Biomedical Research. 15. 287-290 (1994)

Maeda Norihiko：“去神经支配对发育中小鼠咬肌中乳酸脱氢酶同工酶表达的影响。”