Regulation of Cytokines for Gene Expression and Function of Gingival Fibroblasts

细胞因子对牙龈成纤维细胞基因表达和功能的调节

基本信息

批准号：
02670812
负责人：
HANAZAWA Shigemasa
金额：
$ 1.41万
依托单位：
Miekai University School of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670812/
关键词：
Inflammation Cytokines Gingival fibroblasts Periodonal pathogen Periodontal disease Gene expression

项目摘要

Adherence to host cells is an essential stage in triggering the pathogenesis brought about pathogenic bacteria. Bacterial fimbriae may be an important cell structure for the adherence. In many cases, the adherence takes place through the binding of fimbriae to specific receptors on the host cell surface. Therefore, bacterial fimbriae play an important role in first stage of the pathogenesis caused by pathogenic bacteria.Bacteroides (Porphyromonas) gingivalis, an oral anaerobic bacterium, has been recognized widely as a pathogenic organism in adult periodontitis which is a chronic inflammatory disease ; Chemical and immunological characterizations of -B. gingivalis fimbriae have been well demonstrated by Yoshimura et al. We suggested previously that the organism attaches to human fibroblasts through the binding of the fimbriae to specific receptors. Therefore, in the same fashion as adhesion of other gram-negative bacteria, B. gingivalis fimbriae-mediated adhesion is regarded as a first stage in pathogenesis of the microorganism infection.In the present grant-aid for scientific research, we obtained the following results.1) B. gingivalis fimbriae stimulated transcriptional activity of IL-1 beta in mouse peritoneal macrophages.2) The fimbriae induced expression of neutrophil chemoattractant KC gene in mouse peritoneal macrophages via activation of protein kinase C.3) The fimbriae induced IL-6 expression in human gingival fibroblasts, and the expression was mediated in part by membrane IL-1 alpha.4) IL-1 and TNF induced IL-6 expression in human gingival fibroblasts.

遵守宿主细胞是触发致病细菌的发病机理的重要阶段。细菌纤维膜可能是依从性的重要细胞结构。在许多情况下，依从性通过膜的结合与宿主细胞表面上的特定受体的结合发生。因此，细菌纤维膜在致病细菌引起的发病机理的第一阶段起着重要作用。口服厌氧菌（一种口腔厌氧菌）在成人牙周炎中被广泛认为是一种致病性生物体，是一种慢性炎性疾病； -b的化学和免疫学特征。 Yoshimura等人已经很好地证明了牙龈菌。我们先前建议该生物通过纤维膜与特定受体的结合附着在人的成纤维细胞上。因此，以与其他革兰氏阴性细菌的粘附相同的方式，牙龈芽孢杆菌纤维化介导的粘附被认为是微生物感染的发病机理的第一阶段。 Fimbriae通过蛋白激酶的激活在小鼠腹膜巨噬细胞中诱导中性粒细胞促粒细胞Kc基因的表达。3）Fimbriae在人牙龈成纤维细胞中诱导的IL-6表达，该表达在膜IL-1 Alpha.4）IL-1和TNF中介导的部分介导了人类和Tnf ind-6的表达。