Application for diagnosis of B.gingivalis-specific monoclonal antibody in periodontal disease.

牙龈芽孢杆菌特异性单克隆抗体在牙周病诊断中的应用。

基本信息

批准号：
60570876
负责人：
HANAZAWA Shigemasa
金额：
$ 1.09万
依托单位：
Josai dental University, Dept. of Oral Microbiology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

Bacteroides gingivalis is found in the humoral oral environment and has been isolated in relatively high proportions from inflamed gingival pockets of adult periodontal patients. Several studies have indicated that immunoglobulin G serum antibody titers against B.gingivalis are increased significantly in adult periodontal patients. These studies suggest that B.gingivalis may be associated with the pathogenesis of adult periodontal disease. For clinical studies of adult periodontal disease, it is important to rapidly detect and identify B.gingivalis in the oral cavity. Specific and highly sensitive markers of B.gingivalis would be of great use to establishe simple methods for clinical investigation. We have established a established cell line that produces B.gingivalis-specific monoclonal antibody by hybridization technology. Purpose of this study was to evaluate for application of the monoclonal antibody for diagnosis of periodontal disease. An antigen recognized by the monoclonal antibody specific for B.gingivalis was purified by immunoadsorbet column. The purified antigen was homogeneous as jodged by SDS-PAGE and silver staining, and the pattern of wester blotting. The antigen molecule exhibited an apparent molcular weight of about 62,000. A significant positive correlation was observed between seru IgG antibody titer against the specific antigen. However,B.gingivalis was not isolated in significant high frequency from subgingival plaques of adult periodontal patients. Therefore, it is very important to elucidate this discrepancy in feature.

在体液口腔环境中发现了牙龈牙龈牙龈，并且已经从成年牙周病患者的发炎牙龈袋中分离出较高的比例。几项研究表明，成人牙周患者的免疫球蛋白G血清抗体滴度显着增加。这些研究表明，吉维尔斯可能与成人牙周疾病的发病机理有关。对于成人牙周疾病的临床研究，重要的是要快速检测和鉴定口腔中的芽孢杆菌。 B.ghivalis的特定且高度敏感的标志物将极大地用于建立简单的临床研究方法。我们已经建立了一条已建立的细胞系，该细胞系通过杂交技术产生芽孢特异性单克隆抗体。这项研究的目的是评估单克隆抗体诊断牙周疾病的应用。通过免疫adsorbet柱纯化了由芽孢杆菌特异性的单克隆抗体识别的抗原。纯化的抗原是同质的，因为SDS-PAGE和银色染色以及Wester印迹的模式。抗原分子的明显摩尔克量约为62,000。在针对特定抗原的Seru IgG抗体滴度之间观察到显着的阳性相关性。然而，在成年牙周患者的尺寸高频率下，芽孢杆菌并未在显着的高频中分离出来。因此，阐明特征的差异非常重要。