Role of CD14 on human gingival fibroblasts in pathogenesis of periodontal disease

CD14对人牙龈成纤维细胞在牙周病发病机制中的作用

基本信息

批准号：
09671864
负责人：
HANAZAWA Shigemasa
金额：
$ 2.24万
依托单位：
Meikai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Lipopolysaccharide(LPS) of Porphyrom onas gin givalis(P.gin givalis), a pathogenic bacterium of periodontal disease, has been considered an important pathogenic component of the organism in the initiation and development of periodontal diseases, because bacterial LPS is known to be a potent stimulator of inflammatory cytokine production and bone resorption. One target of LPS is the human gingival fibroblast, a cell that play an important role in the remodeling of periodontal soft tissues. These fibroblasts may also function as a regulator of the cytokine network of periodontal tissues, because they produce several kinds of cytokine when stimulated by inflammatory cytokines and bacterial cell components.P.gin givalis LPS stimulates interleukin(IL)-1, IL-8, and IL-6 production by human gingival fibroblasts. More recently, a very interesting study has demonstrated that CD14 gene expression in the mouse is not restricted to myeloid cells only, but also occurs in epithelial cells of various organs. This demonstration suggested to us that LPS maybe involved actively in inflammatory reactions of various tissues via CD14. Therefore, it is very important to examine whether human gingival fibroblasts express CD14 on their cell surface. If they do, it would be of interest to elucidate the CD14-mediated signal transducing mechanism of P.gingivalis LPS in these gingival fibroblasts.In the present study, we demonstrated that human gingival fibroblasts express CDI4 and also demonstrated CD14 mediation of the signal pathway of P.gin givalis LPS in the cells.

斑岩虫（P.gin givalis）的脂多糖（LPS）是一种牙周疾病的一种致病细菌，被认为是生物体在牙周疾病开始和发育中的重要致病成分，因为已知细菌LPS是一种有力的抗炎性刺激剂，是炎性刺激性刺激性刺激性刺激性的。 LPS的一个目标是人牙龈成纤维细胞，该细胞在牙周软组织重塑中起重要作用。这些成纤维细胞也可能是牙周组织细胞因子网络的调节剂，因为当炎症细胞因子和细菌细胞成分刺激时，它们会产生几种细胞因子。最近，一项非常有趣的研究表明，小鼠中的CD14基因表达不仅限于髓样细胞，而且还发生在各种器官的上皮细胞中。该演示向我们表明，LP可能通过CD14积极参与各种组织的炎症反应。因此，检查人牙龈成纤维细胞是否在其细胞表面表达CD14非常重要。如果这样做的话，阐明在这些牙龈成纤维细胞中的CD14介导的信号转导机制。