ATP metabolism in Streptococcus mutans

变形链球菌中的 ATP 代谢

• 批准号: 02670832
• 负责人: KODAMA Takao
• 金额: $ 1.34万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670832/
• 关键词: Streptococcus mutans; ATP generation; ATP utilization; energy metabolism; H^+-ATPase

(1) Streptococcus rattus, a serotype b strain of mutants streptococci, was grown in an anaerobic gulcose-limited chemostat to determine the molar grown yield of glucose, the maximum growth yields and maintenance coefficients form glucose uilizition and calculated ATP generation as a function of pH.Also examined was the presence of an F_1F_0-type H^+-ATPase in cells grown under acidic conditions and in cells grown at neutral pH.The results suggest that the organism responds and adapts to environmental acidification by sacrificing some energy cost in terms of both the efficiency of glucose utilization to generate ATP and the extra maintenance required to continue biomass production ad efficiently as under neutral pH.(2) Kinetic measurements were made of the cellular responses of S.mutans FA-1 to ethanol. Oxygen was absolutely required for acid production from ethanol, which elicited a marked increase in the intracellular ATP concentration (ATP_1) from a starved level to a steady level which was held constant as long as oxygen was present in the medium. Once oxygen was exhausted, ATP_1 returned to the starved level without delay. Both the steady ATP_1 and the rate of accompanying oxygen consumption were functions of the ethanol concentration.

(1)鼠链球菌，一种血清型B菌株的突变链球菌，在厌氧葡萄糖限制恒化器中生长以测定葡萄糖的摩尔生长产率，最大生长产量和维持系数来自葡萄糖利用率和计算的ATP产生量作为pH的函数。还检查了F_1F_0型H^+-的存在。结果表明，在酸性条件下生长的细胞和在中性pH值下生长的细胞中，生物体通过牺牲一些能量成本来响应和适应环境酸化，这两个方面的效率葡萄糖利用产生ATP和ATP酶。需要额外的维护以在中性pH下有效地继续生物质生产。(2)对变形链球菌FA-1对乙醇的细胞反应进行动力学测量。乙醇产酸过程中，氧是必不可少的，只要培养基中有氧存在，细胞内ATP浓度（ATP_1）就会从饥饿水平显著增加到稳定水平。一旦氧气耗尽，ATP_1立即恢复到饥饿水平。稳态ATP_1和伴随的耗氧速率都是乙醇浓度的函数。

项目成果

Miyagi,A.,Ohta,H.,Kodama,T.,Fukui,K.,Kato,K.& Shimono,T.: "Metabolic and energetic aspects of the growth response of Streptococcus rattus to environmental acididification in anaecrobic continuous culture" Microbiology. 140. 1945-1952 (1994)

宫城，A.，太田，H.，儿玉，T.，福井，K.，加藤，K.

宮城 淳,児玉 孝雄,福井 一博,太田 寅行,下野 勉,加藤 慶二郎: "Streptococcus rattusの低pH適応にともなうH^+ーATPaseの誘導" 歯科基礎医学会雑誌. 33. 187 (1991)

Jun Miyagi、Takao Kodama、Kazuhiro Fukui、Torayuki Ota、Tsutomu Shimono、Keijiro Kato：“大鼠链球菌适应低 pH 过程中 H^+-ATP 酶的诱导”日本基础牙科医学会杂志 33. 187（1991 年）。 ）

Miyagi, A., Ohta, H., Kodama, T., Fukui, K., Kato, K., and Shimono, T.: "Metabolic and energetic aspects of the growth response of Streptococcus rattus to environmental acidification in anaerobic continuous culture." Microbiology. 140. 1945-1952 (1994)

Miyagi, A.、Ohta, H.、Kodama, T.、Fukui, K.、Kato, K. 和 Shimono, T.：“厌氧连续培养中鼠链球菌对环境酸化的生长反应的代谢和能量方面

宮城 淳,下野 勉,児玉 孝雄,嶋本 達家,福井 一博,加藤 廣次郎,太田 寛行: "Streptococcus mutansのエタノ-ル好気代謝 V.ATPプ-ルについて" 歯科基礎医学会雑誌. 32. 95 (1990)

Jun Miyagi、Tsutomu Shimono、Takao Kodama、Tatsuie Shimamoto、Kazuhiro Fukui、Hirojiro Kato、Hiroyuki Ota：“关于变形链球菌的有氧乙醇代谢和 V.ATP 库” 日本基础牙科医学会杂志 32. 95。 (1990)