Molecular Energetics of Protein Motors

蛋白质马达的分子能量学

基本信息

  • 批准号:
    06304052
  • 负责人:
  • 金额:
    $ 10.5万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 1996
  • 项目状态:
    已结题

项目摘要

Energetic aspects of protein motors were investigated by various methods including microwave-dielectric spectroscopy and calorimetry of myosin motors hydrolyzind ATP,direct observation of simgle kinesin molecules moving along microtubules, imaging of individual ATP turnovers by single myosin molecules, small-angle X-ray diffeaction of myosin molecules with diffrrent bound nucleotides, X-ray diffraction of muscle, X-ray crystallography of myosin complexed with different nucleotides, diffusion-enhanced fluorescence resonance energy transfer of actin molecules, simulation of solvation free-energy of protein motors, fluctuation analysis of kinesin sliding along microtubule, stereo-photogramtry of quick-freeze deep-etch replica images of motor proteins, and site-directed mutagenesis of motor proteins. These studies indicate that :1.the myosin surface hydrophobicity change plays a crucial role in the enthalpy-entropy compensation effectc observed in the steps of myosin ATP hydrolysis ;2.protein motors can modulate the mode of coupling between chemical change (ATP hydrolysis) and mechanical output depending on load imposed on them. Thus, the energy of ATP hydrolys is somehow stored in the motor protein, which is used fractionally at each power stroke cycle. This would mean that the protein motor operate with energy conversion efficiency as high as more than 50% using the energy input comparable to or marginally above the thermal energy ;3.Of two negative potential sites of actin molecule surface, one around the myosin binding site while the other site around the phalloidin binding site is not significantly affected ;4.an effective diffusion coefficient from displacement fluctuations of a sliding filament obtained from its single noisy trajectory is a useful parameters for constructing the models for mechanochemical coupling.
蛋白质马达的能量方面通过各种方法进行了研究,包括微波介电光谱和肌球蛋白马达水解ATP的量热法,沿沿着微管运动的单个驱动蛋白分子的直接观察,单个肌球蛋白分子的单个ATP周转的成像,肌球蛋白分子与互补结合的核苷酸的小角X射线衍射,肌肉的X射线衍射,肌球蛋白与不同核苷酸复合的X射线晶体学,肌动蛋白分子的扩散增强荧光共振能量转移,蛋白质马达的溶剂化自由能模拟,驱动蛋白沿沿着微管滑动的涨落分析,马达蛋白的快速冷冻深蚀刻复制品图像的立体照相术,以及马达蛋白的定点诱变。这些研究表明:1.肌球蛋白表面疏水性的改变在肌球蛋白ATP水解过程中的熵-熵补偿效应中起着至关重要的作用; 2.蛋白马达可以调节化学变化(ATP水解)和机械输出之间的耦合模式,这取决于施加在它们身上的负载。因此,ATP水解酶的能量以某种方式储存在运动蛋白中,在每个动力冲程周期中部分地使用。3.肌动蛋白分子表面的两个负电位位点中,一个位于肌球蛋白结合位点附近,另一个位于鬼笔环肽结合位点附近,没有受到明显影响; 4.由单噪声轨迹得到的滑移细丝位移涨落的有效扩散系数是建立力化学耦合模型的有用参数。

项目成果

期刊论文数量(47)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
E.Katayama,G.Scott-Woo & M.Ikebe: "Effect of caldesmon on the assembly of smooth muscle myosin" J.Biol.Chem.270. 3319-3925 (1995)
E.片山,G.斯科特-吴
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  • 影响因子:
    0
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  • 通讯作者:
Saeki,K.et al.: "Tropomyosin-Binding Site (s) on the Dictyostelium Actin Surface As Identified by Site-Directed Mutagenesis" Biochemistry. 35. 14465-14472 (1996)
Saeki,K.et al.:“通过定点诱变鉴定的盘基网柄菌肌动蛋白表面上的原肌球蛋白结合位点”生物化学。
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    0
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KODAMA Takao其他文献

KODAMA Takao的其他文献

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{{ truncateString('KODAMA Takao', 18)}}的其他基金

Fundamental and clinical evaluation for the optimization of diagnostic imaging of the temporal bone using 3T MRI
使用 3T MRI 优化颞骨诊断成像的基础和临床评估
  • 批准号:
    23591783
  • 财政年份:
    2011
  • 资助金额:
    $ 10.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Calorimetric and dielectrpspectroscopic studies of energy-transfer mechanism of within a protein molecule
蛋白质分子内能量转移机制的量热和介电光谱研究
  • 批准号:
    13680744
  • 财政年份:
    2001
  • 资助金额:
    $ 10.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Evaluation of focal cerebral perfusion using arterial spin labeling (ASL) technique.
使用动脉自旋标记(ASL)技术评估局灶性脑灌注。
  • 批准号:
    12670889
  • 财政年份:
    2000
  • 资助金额:
    $ 10.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
IS THE RAPID-PHASE OF ENZYME CATALYTIC CYCLES ENTROPY-DRIVEN?
酶催化循环的快速阶段是熵驱动的吗?
  • 批准号:
    06680656
  • 财政年份:
    1994
  • 资助金额:
    $ 10.5万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Research of the scattering process of quasi-particle of superfluid 3He using the fourth sound and the torsional oscillator technique
利用第四声和扭转振子技术研究超流体3He准粒子的散射过程
  • 批准号:
    06452061
  • 财政年份:
    1994
  • 资助金额:
    $ 10.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
ATP metabolism in Streptococcus mutans
变形链球菌中的 ATP 代谢
  • 批准号:
    02670832
  • 财政年份:
    1990
  • 资助金额:
    $ 10.5万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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