Directed evolution used to probe protein structure and function; new enzymes for bio-remediation and industry

用于探测蛋白质结构和功能的定向进化；

• 批准号: DP0342678
• 负责人: Prof David Ollis
• 金额: $ 16.75万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2006-07-05
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0342678
• 关键词: Directed; evolution; used; probe; protein

The aim of the research is to generate new and useful enzymes for bio-remediation and other practical applications. For example, we are evolving enzymes to better degrade organophosphate pesticides that are environmental pollutants. Apart from producing useful enzymes, the proposed research aims at gaining a better understanding of how enzymes work and how they evolve. We intend to determine the structure of many related enzymes that have been evolved to have enhanced activities. This data will be used to analyze the intricate relationship between sequence, structure and enzyme activity.

该研究的目的是产生新的和有用的酶用于生物修复和其他实际应用。例如，我们正在开发酶，以更好地降解环境污染物有机磷农药。除了生产有用的酶外，拟议的研究旨在更好地了解酶如何工作以及它们如何进化。我们打算确定许多相关酶的结构，这些酶已经进化到具有增强的活性。这些数据将用于分析序列，结构和酶活性之间的复杂关系。