IMMUNOLOGICAL AND MOLECULAR STUDIES ON SECRETORY IGA

分泌型 IGA 的免疫学和分子研究

• 批准号: 03404052
• 负责人: MORO Itaru
• 金额: $ 10.37万
• 依托单位: NIHON UNIVERSITY SCHOOL OF DENTISTRY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03404052/
• 关键词: Secretory IgA; Secretory Component; Joining Chain; Cytokine; Epithelial Cell; Polymerase Chain Reaction; Northern Blot; Western Blot; Polymeric IgA; 脊椎動物; 無脊椎動物; PCR法; Northern blot法; Secretory-Component; Interleukin-6; Tumor Necrosis Facto

The results obtained by this study supported by The Ministry of Education, Science and Culture, Grant-in-Aid for Scientific Research (A) were summarized below ;1 .Development and localization of Joining (J) chain, secretory component (SC), IgA and IgM in human fetus(1) a significant reactivity of J chain expression was found at the 6 th gestational week and the expression of mu chain at week 7 laged behind that of J chain in the early fetal liver.(2) both J chain and mu chain were detected at the 16 th gestational week in spleen, thymus, small intestine and lung.(3) SC and alpha chain could not detect until the 16 th gestational week in all organs examined.2. Expression of cytokine and SC in colonic epithelial cell line HT-29(1) HT-29 stimulated with Salmonella minesota LPS resulted in the expression of IL-6, IL-8, TGF-beta1 and SC mRNA.(2) up-regulation of SC mRNA and protein was detected in HT-29 stimulated with IFN-gamma, TNF-alpha, IL-1alpha, IL-1beta, IL-4 or IL-6.(3) presence of IL-1 receptor type I in HT-29 was suggested.3. Phylogeny of J chain(1) invertebrates that do not produce immunoglobulins have the J chain mRNA similar to vertebrates.(2) earthworm (Eisenia faetida) J chain cDNA as well as amino acid sequence has a high degree of homnology (75%, 76%) to human and mouse, respectively.(3) J chain protein in earthworm and slug was localized in the epithelial cells of skin and small intestine.(4) these results suggested that the real function of J chain may not be a polymerization of immunoglobulin.

这项研究得到了教育、科学和文化部科学研究补助金（A）的支持，其结果概述如下：1 .连接（J）链、分泌成分（SC）、人胎儿伊加和IgM（1）J链在孕6周时表达明显，而μ链在孕7周时表达滞后于J链，早期胎儿肝脏(2)孕16周时，脾脏、胸腺、小肠和肺中均检测到J链和μ链。(3)SC和α链在所有检查的器官中直到孕16周才检测到. HT-29结肠上皮细胞系中细胞因子和SC的表达（1）用Salmonella minesota LPS刺激HT-29导致IL-6、IL-8、TGF-β 1和SC mRNA的表达。(2)在用IFN-γ、TNF-α、IL-1 α、IL-1 β、IL-4或IL-6刺激的HT-29中检测到SC mRNA和蛋白的上调。(3)证实HT-29细胞中存在I型IL-1受体. J链的系统发育（1）不产生免疫球蛋白的无脊椎动物具有与脊椎动物相似的J链mRNA。(2)其J链cDNA和氨基酸序列与人和小鼠的同源性分别为75%和76%。(3)蛞蝓和蛞蝓的J链蛋白定位于皮肤和小肠上皮细胞。(4)这些结果提示J链的真实的功能可能不是免疫球蛋白的聚合。

项目成果

Itaru MORO et al.: "Ontogeny of the Secretory IgA System in Humans" Adv.Exp.Med.Biol.310. 51-57 (1991)

Itaru MORO 等人：“人类分泌型 IgA 系统的个体发育”Adv.Exp.Med.Biol.310。

MORO ITARU: "ONTOGENY OF THE SECRETORY IgA SYSTEM IN HUMANS" ADV.EXP.MED.BIOL.310. 51-57 (1991)

Moro Itaru：“人类分泌型 IgA 系统的个体发育”ADV.EXP.MED.BIOL.310。

TSUCHIYA MASAHARU: "FRONTIERS OF MUCOSAL IMMUNOLOGY Vol.2" Elsevier Science Publishers B.V., 660 (1991)

土屋雅晴：“粘膜免疫学前沿第 2 卷”Elsevier Science Publishers B.V.，660 (1991)

ITARU MORO,KAZUO KOMIYAMA,KAORU KUSAMA,TOMIHISA TAKAHASHI AND NOBUKO TAKENOUCHI: "SECRETORY IMMUNOGLOBULIN" GI RESEARCH. 1. 22-28 (1993)

Itaru Moro、Kazuo KOMIYAMA、Kaoru Kusama、Tomihisa Takahashi 和 Nobuko TAKENOUCHI：“分泌型免疫球蛋白”GI 研究。

TAKAHASHI TOMIHISA: "JOINING (J) CHAIN IS PRESENT IN INVERTEBRATESTHAT DO NOT EXPRESS IMMUNOGLOBULINS." (発表予定).

高桥富久：“连接（J）链存在于不表达免疫球蛋白的无脊椎动物中。”（待公布）。