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Study on development of new bactericidal-biocompatible material for conserving infected pulp.

新型杀菌生物相容性牙髓保存材料的开发研究

基本信息

批准号：
03404055
负责人：
IWAKU Masaaki
金额：
$ 20.42万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1994
项目状态：
已结题

项目摘要

This study was projected for developing the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.Following results were obtained :1. A mixture of Ciprofloxacin, Metronidazole and Cefaclor showed the complete bactericidal efficacy against all of the bacteria in the infected pulp tissue.2. The antibacterial mechanism of metronidazole was revealed the investigation of the metabolism of phenylalanine and leucine of anaerobic bacteria.3. alpha-tricalcium phosphate (alpha-TCP) showed the superior biocompatibility as the direct capping material on the histopathological study using adult monkeys.4. The histopathological study showed that alpha-TCP containing mixed antibacterial drugs effectively disinfected the bacterially contaminated pulpal lesions of adult monkeys without destroying any of the sound pulp tissue and formed the hard tissue barrier covering the exposed pulpal surface.5. alpha-TCP containing mixed antibacterial drugs completely disinfected human caries lesions in vitro and in situ studies.6. In the in vivo study of the volunteer peoples, artificially exposed pulp tissue was convered by the hard tissue barrier formation without destroying any of the sound pulp tissue, when alpha-TCP containing mixed antibacterial drugs was applied on the exposed pulp tissue.7. In the clinical study of the volunteer patients, almost cases of the infected pulp, especially young, has been kept without any of the pulpectomy or pulpotomy by the application of the mixed antibacterial drugs added to alpha-TCP and covered with time by the hard tissue barrier.8. This work is now in progress.

本研究旨在开发最有效的新型杀菌生物相容性材料来保存受感染的牙髓组织。获得以下结果： 1．环丙沙星、甲硝唑和头孢克洛的混合物对感染牙髓组织中的所有细菌均具有完全的杀菌功效。 2．通过对厌氧菌苯丙氨酸和亮氨酸代谢的研究，揭示了甲硝唑的抗菌机制。 3．在成年猴的组织病理学研究中，α-磷酸三钙(α-TCP)作为直接封盖材料显示出优异的生物相容性。 4．组织病理学研究表明，含有混合抗菌药物的α-TCP可有效消毒成年猴被细菌污染的牙髓病灶，且不破坏任何健全的牙髓组织，并形成覆盖暴露牙髓表面的硬组织屏障。5．含α-TCP的混合抗菌药物对人体龋损彻底消毒的体外和原位研究。 6．在志愿者的活体研究中，当在暴露的牙髓组织上涂抹含有混合抗菌药物的α-TCP时，人工暴露的牙髓组织被硬组织屏障的形成所恢复，而不破坏任何健全的牙髓组织。 7．在志愿者患者的临床研究中，几乎所有感染牙髓的病例，特别是年轻患者，都通过使用添加到α-TCP的混合抗菌药物并通过硬组织屏障随着时间的推移而覆盖，而没有进行任何牙髓切除或活髓切断术。8．这项工作目前正在进行中。