Study on development of new bactericidal-biocompatible material for conserving infected pulp.

本研究旨在开发一种最有效的新型杀菌-生物相容材料来保存受感染的牙髓组织。获得的结果如下：1.环丙沙星、甲硝唑和头孢克洛的混合物对感染牙髓组织中的所有细菌都具有完全的杀菌效果。通过对厌氧菌苯丙氨酸和亮氨酸代谢的研究，揭示了甲硝唑的抗菌机理。在成年猴的组织病理学研究中，α-磷酸三钙(α-TCP)作为直接盖髓材料显示出了良好的生物相容性。组织病理学研究表明，含有混合抗菌药物的α-磷酸三钙有效地消毒了被细菌污染的成年猴牙髓病变，而不破坏任何健康的牙髓组织，并形成了覆盖在暴露的牙髓表面的硬组织屏障。含α-磷酸三钙混合抗菌药物对人体龋损完全消毒的体外和原位研究。在志愿者的活体研究中，人工暴露的牙髓组织在不破坏任何健康牙髓组织的情况下，通过硬组织屏障的形成被转化，而暴露的牙髓组织上应用含有混合抗菌药物的α-磷酸三钙。在志愿患者的临床研究中，通过在α-磷酸三钙中加入混合抗菌药物，并用硬组织屏障覆盖时间，几乎所有感染的牙髓病例，特别是年轻人，在没有进行任何牙髓切除或切髓的情况下被保存下来。这项工作目前正在进行中。

This study was projected for developing the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.Following results were obtained :1. A mixture of Ciprofloxacin, Metronidazole and Cefaclor showed the complete bactericidal efficacy against all of the bacteria in the infected pulp tissue.2. The antibacterial mechanism of metronidazole was revealed the investigation of the metabolism of phenylalanine and leucine of anaerobic bacteria.3. alpha-tricalcium phosphate (alpha-TCP) showed the superior biocompatibility as the direct capping material on the histopathological study using adult monkeys.4. The histopathological study showed that alpha-TCP containing mixed antibacterial drugs effectively disinfected the bacterially contaminated pulpal lesions of adult monkeys without destroying any of the sound pulp tissue and formed the hard tissue barrier covering the exposed pulpal surface.5. alpha-TCP containing mixed antibacterial drugs completely disinfected human caries lesions in vitro and in situ studies.6. In the in vivo study of the volunteer peoples, artificially exposed pulp tissue was convered by the hard tissue barrier formation without destroying any of the sound pulp tissue, when alpha-TCP containing mixed antibacterial drugs was applied on the exposed pulp tissue.7. In the clinical study of the volunteer patients, almost cases of the infected pulp, especially young, has been kept without any of the pulpectomy or pulpotomy by the application of the mixed antibacterial drugs added to alpha-TCP and covered with time by the hard tissue barrier.8. This work is now in progress.