Study on development and clinical application of new bactericidal-biocompatible material for conserving infected pulp.

新型杀菌生物相容性牙髓保存材料的开发及临床应用研究

• 批准号: 08407063
• 负责人: IWAKU Masaaki
• 金额: $ 20.74万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08407063/
• 关键词: Infected Pulp; Obligate Anaerove; Metoronidazol; alpha-Tricalcium phosphate; Biocompatibility; Antibacterial Drug; Hard Tissure Barrier; Pulp Conservation

This study was projected for development and clinical application of the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.Following results were obtained :1. RFLP Analysis of 16S rDNA from Pseudolamibacter alactolyticus.In this study, we examined the restriction fragment-length polymorphism(RFLP) of the 16S-rDNA amplified by poymerase chain reaction(PCR) to assess phylogenetic heterogeneity of this bacterial species. In conclusion, these data suggested that P.alactolyticus may not be a phylogenetically homogeneous species.2. Artificial Bacterial Bioflims and Confocal Laser Scanning Microscopic Analysis.To elucidate the structure and the function of biofilms, mucoid-type Pseudomonas aeruginosa organisms were cultured in broth media supplied. The biofilm structure was visualized by fluorescent staining and were exposed to ciprofloxacin.These results indicate that the confocal laser scanning microscopical analysis of artificial biofilms was useful to elucidate bacterial functions in bioflims, and may lead to new quantitative estimation of bactericidal efficacy of natibacterial drugs.3. Tooth development is regulated by epithelial-mesenchymal interactions mediated by the basement membrane. The expression and localization of laminin-5 were temporally and spatially regulated during mouse tooth development. The temporo-spatial changes in basement membrane composition might be related with tooth morphogenesis and cytodifferentiation.4. The amount of fluoride release from fluoridated restorative materials, fluoride uptake into enamel and dentine, and effect of fluoride on acid resistance of the tooth structures, were evaluated Fluoride releasing materials was effective for prevention of secondary caries.5.This work is now in progress

本研究旨在开发和临床应用最有效的新型杀菌-生物相容材料来保存感染的牙髓组织。获得的结果如下：1.溶乳假单胞菌16S rDNA的RFLP分析。综上所述，这些数据提示溶半乳杆菌可能不是一个系统发育上同源的物种。人工细菌生物膜和激光共聚焦扫描显微镜分析为了阐明生物膜的结构和功能，在提供的肉汤中培养粘液型铜绿假单胞菌。人工生物膜的激光共聚焦扫描显微镜分析有助于阐明生物膜中细菌的功能，并可能导致对国产药物杀菌效果的新的定量评估。牙齿发育受基底膜介导的上皮-间充质相互作用的调节。在小鼠牙齿发育过程中，层粘连蛋白-5的表达和定位受到时间和空间的调控。基底膜成分的时空变化可能与牙齿形态发生和细胞分化有关。对含氟修复材料的氟化物释放量、牙釉质和牙本质的氟化物摄取量以及氟化物对牙齿结构耐酸性的影响进行了评价。5.含氟修复材料对预防继发性龋病的有效性

项目成果

Kunihiko Yoshiba: "Expression and localization of laminin-5 subunits during mouse tooth development." Developmental Dynamics. 211. 164-176 (1998)

Kunihiko Yoshiba：“小鼠牙齿发育过程中层粘连蛋白 5 亚基的表达和定位。”

韓 臨麟et al: "接着性コンポジットレジンにおけるフッ素含有フィラー配合ボンディング材に関する研究-フッ素徐放性,歯質への取り込みおよびその歯質耐酸性に及ぼす影響-" 日歯保存誌. 41巻. 690-697 (1998)

Lin-Lin Han 等人：“粘合复合树脂中含有含氟填料的粘合材料的研究 - 牙齿结构中氟化物的持续释放、吸收及其对牙齿结构耐酸性的影响 -”《牙科保护杂志》第 41 卷。 690 -697 (1998)

N.Yoshiba: "Immunolocalization of the small proteoglycan decorin in human teeth." Archs oral Biol.41(4). 351-357 (1996)

N.Yoshiba：“人类牙齿中小蛋白聚糖核心蛋白聚糖的免疫定位。”

Kunihiko Yoshiba et al: "Expression and localization of laminin-5 subunits during mouse tooth development" Developmental Dynamics. 211巻. 164-176 (1998)

Kunihiko Yoshiba 等人：“小鼠牙齿发育过程中层粘连蛋白 5 亚基的表达和定位”，Developmental Dynamics，第 211 卷，164-176（1998 年）。

Nagako Yoshiba: "Immunohistochemical Localization of Class II Antigens and Nerve Fibers in Human Carious Teeth : HLA-DR Immunoreactivity in Schwann Cells" Arch.Histol.Cyto.Vol.61 (4). 343-352 (1998)

Nagako Yoshiba：“人类龋齿中 II 类抗原和神经纤维的免疫组织化学定位：雪旺细胞中的 HLA-DR 免疫反应性”Arch.Histol.Cyto.Vol.61 (4)。