Study on development and clinical application of new bactericidal-biocompatible material for conserving infected pulp.

本研究旨在开发最有效的新型杀菌生物相容性材料，以保存受感染的牙髓组织。得到了以下结果：1。产乳酸假菌16S rDNA的RFLP分析。在这项研究中，我们检测了聚合酶链反应（PCR）扩增的16S-rDNA的限制性片段长度多态性（RFLP），以评估该细菌物种的系统发育异质性。综上所述，这些数据表明，乳酸乳酸杆菌可能不是一个系统发育上一致的物种。人工细菌生物膜及共聚焦激光扫描显微分析。为了阐明生物膜的结构和功能，在提供的肉汤培养基中培养了粘液型铜绿假单胞菌。荧光染色显示生物膜结构，并暴露于环丙沙星。这些结果表明，激光扫描显微共聚焦分析人工生物膜有助于阐明细菌在生物膜中的功能，并可为抗菌药物的杀菌效果提供新的定量评价方法。牙齿的发育受基膜介导的上皮-间质相互作用的调节。在小鼠牙齿发育过程中，laminin-5的表达和定位受到时间和空间的调控。基底膜组成的时空变化可能与牙齿的形态发生和细胞分化有关。评价了氟化修复材料的氟释放量、氟在牙釉质和牙本质的吸收以及氟对牙结构耐酸性的影响。这项工作目前正在进行中

This study was projected for development and clinical application of the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.Following results were obtained :1. RFLP Analysis of 16S rDNA from Pseudolamibacter alactolyticus.In this study, we examined the restriction fragment-length polymorphism(RFLP) of the 16S-rDNA amplified by poymerase chain reaction(PCR) to assess phylogenetic heterogeneity of this bacterial species. In conclusion, these data suggested that P.alactolyticus may not be a phylogenetically homogeneous species.2. Artificial Bacterial Bioflims and Confocal Laser Scanning Microscopic Analysis.To elucidate the structure and the function of biofilms, mucoid-type Pseudomonas aeruginosa organisms were cultured in broth media supplied. The biofilm structure was visualized by fluorescent staining and were exposed to ciprofloxacin.These results indicate that the confocal laser scanning microscopical analysis of artificial biofilms was useful to elucidate bacterial functions in bioflims, and may lead to new quantitative estimation of bactericidal efficacy of natibacterial drugs.3. Tooth development is regulated by epithelial-mesenchymal interactions mediated by the basement membrane. The expression and localization of laminin-5 were temporally and spatially regulated during mouse tooth development. The temporo-spatial changes in basement membrane composition might be related with tooth morphogenesis and cytodifferentiation.4. The amount of fluoride release from fluoridated restorative materials, fluoride uptake into enamel and dentine, and effect of fluoride on acid resistance of the tooth structures, were evaluated Fluoride releasing materials was effective for prevention of secondary caries.5.This work is now in progress