Study on development and clinical application of new bactericidal-biocompatible material for conserving infected pulp.

本研究旨在开发一种新型的、最有效的、具有生物相容性的牙髓组织保存材料并应用于临床。本研究采用聚合酶链反应（PCR）技术对非溶乳假单胞菌（Pseudolamibacter alactolyticus）的16 S rDNA进行限制性片段长度多态性（RFLP）分析，以评价该菌的系统发育异质性。因此，这些数据表明，解乳毕赤酵母可能不是一个遗传上均一的种.人工细菌生物膜和共聚焦激光扫描显微镜分析为了阐明生物膜的结构和功能，在提供的肉汤培养基中培养粘液型铜绿假单胞菌。结果表明，共聚焦激光扫描显微镜分析人工生物膜有助于阐明生物膜中细菌的功能，并可能为天然抗菌药物杀菌效果的定量评价提供新的思路.牙齿的发育受基底膜介导的上皮-间充质相互作用的调节。层粘连蛋白-5在小鼠牙齿发育过程中的表达和定位受到时间和空间的调控。基底膜成分的时空变化可能与牙齿的形态发生和细胞分化有关.评价了含氟修复材料的氟释放量、牙釉质和牙本质的氟吸收量以及氟对牙体结构抗酸能力的影响。结果表明，含氟修复材料对预防继发龋是有效的

This study was projected for development and clinical application of the most effective new bactericidal-biocompatible material to conserve the infected pulp tissue.Following results were obtained :1. RFLP Analysis of 16S rDNA from Pseudolamibacter alactolyticus.In this study, we examined the restriction fragment-length polymorphism(RFLP) of the 16S-rDNA amplified by poymerase chain reaction(PCR) to assess phylogenetic heterogeneity of this bacterial species. In conclusion, these data suggested that P.alactolyticus may not be a phylogenetically homogeneous species.2. Artificial Bacterial Bioflims and Confocal Laser Scanning Microscopic Analysis.To elucidate the structure and the function of biofilms, mucoid-type Pseudomonas aeruginosa organisms were cultured in broth media supplied. The biofilm structure was visualized by fluorescent staining and were exposed to ciprofloxacin.These results indicate that the confocal laser scanning microscopical analysis of artificial biofilms was useful to elucidate bacterial functions in bioflims, and may lead to new quantitative estimation of bactericidal efficacy of natibacterial drugs.3. Tooth development is regulated by epithelial-mesenchymal interactions mediated by the basement membrane. The expression and localization of laminin-5 were temporally and spatially regulated during mouse tooth development. The temporo-spatial changes in basement membrane composition might be related with tooth morphogenesis and cytodifferentiation.4. The amount of fluoride release from fluoridated restorative materials, fluoride uptake into enamel and dentine, and effect of fluoride on acid resistance of the tooth structures, were evaluated Fluoride releasing materials was effective for prevention of secondary caries.5.This work is now in progress