Characterization of structural and regulatory genes of rice nitrite reductase.

水稻亚硝酸还原酶结构和调控基因的表征。

• 批准号: 03660084
• 负责人: IDA Shoji
• 金额: $ 1.34万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660084/
• 关键词: Rice; Oryza sativa L; Nitrite reductase; cDNA library; cDNA sequence; cDNA with an intervening sequence; 分子生物学; 亜硝酸レダクタ-ゼ; 亜硝酸レダクタ-ゼcDNA; 亜硝酸レダクタ-ゼcDNAの塩基配列

Leaf and root cDNA libraries were constructed in lambdagt 11 phage vector from nitrate-induced greening rice seedlings. Two different types of nitrite reductase (NiR) cDNA from each library were cloned and sequenced. DNA sequence of one group had an open reading frame matched with the isolated NiR protein sequence, while cDNA clone from the other had an addtional sequence of 93 bp in the reading frame of the former clone. The insertion sequence has consensus sequence of 5' and 3' splice junction in eukaryotic mRNA. This inserted DNA sequence was confirmed to exist in rice nuclear genomic DNA by PCR technique using the genomic DNA as a template. Complete sequencing of the rice NiR gene may provide a clue to elucidating physiological significance of this unusual presence of an intervening sequence in NiR cDNA.

以硝酸盐诱导的水稻幼苗为材料，在lambdagt 11噬菌体载体上构建了叶片和根系cDNA文库。从每个文库中克隆出两种不同类型的亚硝酸盐还原酶（NiR） cDNA并进行测序。其中一组DNA序列有一个与分离的近红外蛋白序列匹配的开放阅读框，而另一组cDNA克隆在前一组克隆的阅读框中有一个93 bp的额外序列。该插入序列在真核mRNA中具有5′和3′剪接的一致序列。以水稻基因组DNA为模板，采用PCR技术证实该插入序列存在于水稻核基因组DNA中。水稻近红外基因的完整测序可能为阐明这种不寻常的中间序列在近红外cDNA中存在的生理意义提供线索。

项目成果

S.IDA: "The presence of an interbening sequence in a rice ferredoxin-nitrite reductase cDNA."

S.IDA：“水稻铁氧还蛋白-亚硝酸盐还原酶 cDNA 中存在插入序列。”

S. Ida, Y. Terada and H. Aoki: "Cloning and nucleotide sequence of a rice ferrdoxin-nitrite reductase cDNA containing an intervening sequence." Nippon Nogeikagaku Kaishi. 67. 232 (1993)

S. Ida、Y. Terada 和 H. Aoki：“含有插入序列的水稻铁氧还蛋白-亚硝酸盐还原酶 cDNA 的克隆和核苷酸序列。”

S.Ida: "Cloning and cDNA sequence of rice root ferredoxinーNADP^+ reductase"

S.Ida：“水稻根铁氧还蛋白－NADP^+还原酶的克隆及cDNA序列”

青木 秀之: "イネフェレドキシン-NAPP^+レダクターゼの器官特異的cDNAのクローニングと塩基配列" 日本農芸化学会誌. 67. 232 (1993)

Hideyuki Aoki：“水稻铁氧还蛋白-NAPP^+还原酶的器官特异性 cDNA 的克隆和碱基序列”日本农业化学学会杂志 67. 232 (1993)。

青木 秀之: "イネフェレドキシン-NADP^+レダクターゼの器官特異的cDNAのクローニングと塩基配列" 67. 232- (1993)

Hideyuki Aoki：“水稻铁氧还蛋白-NADP^+还原酶的器官特异性 cDNA 的克隆和核苷酸序列”67. 232- (1993)