Molecular analysis of two ethylene-induced cell death processes in rice (Oryza sativa L.)

水稻 (Oryza sativa L.) 中两种乙烯诱导的细胞死亡过程的分子分析

• 批准号: 143567865
• 负责人: Privatdozentin Dr. Bianka Steffens
• 金额: --
• 依托单位: Zentrum für Molekularbiologie der Pflanzen (ZMBP)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/143567865?language=en
• 关键词: Molecular; analysis; two; ethylene; induced

Cell death is an intrinsic part of plant development. It can be developmentally regulated or be induced by environmental signals. Flooded plants suffer from lack of oxygen. To avoid hypoxic stress, well adapted plants such as rice improve their oxygen supply through growth of roots at the stem and through formation of internal air spaces (aerenchyma). Growth of roots is preceded by death of epidermal cells that cover the root primordia. Epidermal cell death as well as aerenchyma formation are regulated by the plant hormone ethylene. We previously identified morphological and molecular differences between epidermal cells that undergo cell death and those that do not. In the current project we attempt to define the cellular characteristics and the signaling pathway of epidermal cell death. We will functionally characterize genes that were found to be differentially active in dying versus non-dying cells with a focus on genes which determine cell identity. Such genes may be responsible for the ability to undergo cell death and for the local confinement of cell death. With the help of mutants, genes that have been recognized as being important for epidermal cell death will be studied in aerenchyma formation, which represents a second ethylene-induced local cell death process. This comparative part of the study should help pinpoint common and diverging pathways in the signaling and execution of cell death by ethylene.

细胞死亡是植物发育的一个内在组成部分。它可以由发育调节或由环境信号诱导。被水淹没的植物因缺氧而受害。为了避免低氧胁迫，适应性好的植物如水稻通过茎部的根的生长和通过内部空气空间（通气组织）的形成来改善它们的氧气供应。根的生长是以覆盖根原基的表皮细胞死亡为先导的。表皮细胞死亡以及通气组织的形成受植物激素乙烯的调节。我们以前确定的表皮细胞进行细胞死亡和那些没有形态和分子差异。在本项目中，我们试图定义表皮细胞死亡的细胞特征和信号通路。我们将在功能上表征被发现在垂死细胞与非垂死细胞中具有差异活性的基因，重点是决定细胞身份的基因。这些基因可能负责经历细胞死亡的能力和细胞死亡的局部限制。在突变体的帮助下，已被认为是重要的表皮细胞死亡的基因将在通气组织的形成，这代表了第二次乙烯诱导的局部细胞死亡过程进行研究。这项研究的比较部分应该有助于查明共同和分歧的途径，在信号和执行细胞死亡的乙烯。