STUDIES ON THE CLEANUP METHOD OF ORGANO CHLORINATED COMPOUND CONTAMINATED SOILS BY MICROORGANISMS

微生物净化有机氯化物污染土壤方法的研究

• 批准号: 03660121
• 负责人: YAGI Osami
• 金额: $ 1.6万
• 依托单位: NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660121/
• 关键词: VOLATILE ORGANOCHLORINATED COMPOUNDS; SOIL CLEANUP; GROUNDWATER POLLUTION; TRICHLOROETHYLENE; TETRACHLOROETHYLENE

A methane-utilizing bacterium, Methylocystis sp. M (strain M) was isolated from soil. This strain degrades trichloroethylene(TCE). Strain M can degrade TCE at a relatively high concentration fo 35 mg/L and as well as various halogenated aliphatic compounds. Bioremediation of TCE-contaminated soil by strain M was investigated in soil microcosms, using 70 ml of serum bottles and 450-ml glass columns as the soil microcosms. Strain M was added to the microcosms filled with a TCE-contaminated soil and water mixture with TCE concentration ranging from 0.1 to 1 ppm. More than 95% of TCE of 0.1 ppm was degraded by the addition of strain M, and degradation of 1,1-, cis- and trans-dichloroethylene (DCE) was accelerated by the strain. H_2O_2 addition with strain M significantly promoted TCE degradation. Strain M proved to be very effective to cleaning up the TCE-contaminated soil.

从土壤中分离到一株甲烷利用细菌甲基孢囊菌（Methylocystis sp. M）。该菌株降解三氯乙烯（TCE）。菌株M能降解35 mg/L的三氯乙烯，并能降解多种卤代脂肪族化合物。采用70 ml血清瓶和450 ml玻璃柱作为土壤微宇宙，研究了菌株M对三氯乙烯污染土壤的生物修复作用。将菌株M添加到充满TCE污染的土壤和水混合物的微观世界中，TCE浓度范围为0.1至1 ppm。菌株M对0.1ppm三氯乙烯的降解率达95%以上，对1，1-、顺式和反式二氯乙烯（DCE）的降解均有促进作用。H_2O_2的加入显著促进了菌株M对三氯乙烯的降解。菌株M对三氯乙烯污染的土壤具有很好的净化效果。

项目成果

矢木 修身、内山 裕夫: "揮発性有機塩素化合物の微生物による分解・除去" 水環境学会誌. 15. 493-498 (1992)

Osumi Yagi、Hiroo Uchiyama：“挥发性有机氯化合物的微生物分解和去除”日本水环境学会杂志 15. 493-498 (1992)。

松井 三郎、矢木 修身 他: "環境微生物工学研究法" 技報堂, 450 (1993)

松井三郎、八木大美等：《环境微生物工程研究方法》Gihodo，450（1993）

谷吉樹、矢木修身他: "バイオコンバージョン" 医学出版センター, 250 (1993)

Yoshiki Tani、Osami Yagi 等：“Bioconversion”医学出版中心，250 (1993)

Uchiyama, H., Nakajima, T., Yagi, O.and Nakahara, T.: "Role of heterotrophic bacteria in complete mineralization of trichloroethylene by Methylocystis sp.M" Appl.Environ.Microbiol.58. 3067-3071 (1992)

Uchiyama, H.、Nakajima, T.、Yagi, O. 和 Nakahara, T.：“异养细菌在甲基囊藻完全矿化三氯乙烯中的作用”Appl.Environ.Microbiol.58。

Nakajima, T., Uchiyama, H., Yafi, O.and Nakahara, T.: "Novel metabolite of trichloroethylene in a methanotrophic bactreium, Methylocystis sp.M, and hypothetical degradation pathway" Biosci.Biotech.Biochem.56. 486-489 (1992)

Nakajima, T.、Uchiyama, H.、Yafi, O. 和 Nakahara, T.：“甲烷氧化细菌、甲基囊藻 sp.M 中三氯乙烯的新代谢物和假设的降解途径”Biosci.Biotech.Biochem.56。