Control of anaerobic organo-chlorinated compound degrading mixed culture under restricted hydrogen condition and its application for bioremediation

限氢条件下厌氧有机氯化合物降解混合培养物的控制及其生物修复应用

• 批准号: 18380191
• 负责人: YAGI Osami
• 金额: $ 6.5万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380191/
• 关键词: cis-DCE; vinyl chloride; chloroethvlene; Dehalococcoides; dehaloeenation; volatile organo-chlorinated compound; 揮発性有機塩素化合物; 嫌気性微生物; 13C-SIP法; 有害化学物質

The purpose of this research is to construct chloroethene degrading mixed culture and to determine the degradation characteristics at various hydrogen concentrations and its application for bioremediation. A chloroethene degrading enrichment culture was constructed and its biodegradation characteristics were investigated. The bacterial source of the highly enriched culture was a lotus field soil. cis-DCE of 10mg/1 was completely degraded to ethene in 21 days by the enrichment culture. The high degradation activity was kept by the addition of 5% of the lotus field soil. The number of Dehalococcoides was kept at 10^<6-7> copies/ml. In organic acids, especially lactate significantly accelerated the cis-DCE degradation activity. The degradation rate increased by the addition of VBI.2. The low ORP and neutral pH were also important factors to achieve the high degradation activity. The hydrogen concentration was a very important factor to keep high dechlorination rate. cis-DCE was degraded to vinyl chloride at the hydrogen concentration less than10^4ppmv revel and VC was degraded to ethylene less than 10^3ppmv revel. In order to keep the high degradation rate of contaminating chloroethene, to control the low hydrogen, the low organic acid and the high VB^<12> concentrations were very important factors.

本研究旨在构建氯乙烯降解混合菌，研究其在不同氢气浓度下的降解特性及其在生物修复中的应用。构建了氯乙烯降解富集菌，并对其生物降解特性进行了研究。高度富集培养物的细菌来源是莲田土壤。10 mg/l的顺式DCE经富集培养21天后完全降解为乙烯。添加5%的莲田土可保持较高的降解活性。脱卤球菌的数量保持在10 μ<6-7>拷贝/ml。在有机酸中，乳酸盐显著促进了顺式DCE的降解。添加VBI.2后，降解速率加快。低氧化还原电位和中性pH也是获得高降解活性的重要因素。氢气浓度是保证高脱氯率的重要因素。当氢气浓度小于10^4ppmv revel时，顺式二氯乙烯降解为氯乙烯，VC降解为乙烯。为了保持高的氯乙烯降解率，控制低氢、低有机酸和高<12>VB_2浓度是非常重要的因素。

项目成果

クロロエチレン分解コンソーシアの脱塩素化活性に及ぼす酸化還元電位の影響

氧化还原电位对氯乙烯分解聚团脱氯活性的影响

• 发表时间: 2007
• 作者: 矢木 修身;他
• 通讯作者: 他

土壌中からの新規クロロエチレン分解菌の探索

寻找土壤中新的氯乙烯降解细菌

• 发表时间: 2008
• 作者: 矢木 修身;他
• 通讯作者: 他

Effect of Hydrogen Concentration on the Chloroethene Dechlorination in Anaerobic Soil Microcosms

氢气浓度对厌氧土壤微环境中氯乙烯脱氯的影响

• 发表时间: 2008
• 作者: Yagi Osami;et. al.
• 通讯作者: et. al.