权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on the Water Bloom of Toxic Alga, Microcystis Viridis

有毒藻类绿色微囊藻水华的研究

基本信息

批准号：
63560115
负责人：
YAGI Osami
金额：
$ 1.28万
依托单位：
The National Institute for Environmental Studies
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63560115/
关键词：
Microcystis Anabaena Blue-green alga Water bloom Growth Growth stimulate compound Ligand Copper Chlorella 比増殖速度増殖特性

项目摘要

We studied the water bloom of toxic alga Microcystis viridis in the field and laboratory experiment. A field study was carried out from summer to fall between 1988-1990 to clarify the change in the dominant species of Microcystis in Lake Kasumigaura. M. aeruginosa f. aeruginosa and M. flos-aquae were dominant in te summer, but M. viridis became dominant in the fall. A high correlation was found between the standing crop of M. aeruginosa and the water temperature, but the standing croup of M. viridis and M. wesenbergii showed no correlation in the water temperature. Effects of temperature and illumination on the specific growth rates and the algal yield of M. viridis N-1 and M. viridis NIES 102 were studied with a batch culture. Maximum specific growth rates of M. viridis N-1 and M. viridis NIES 102 were observed at 25 and 30^0C, respectively. M. viridis could grow at lower temperatures than the other species of Microcystis. M. viridis N-1 could not grow at the continuous illumination o … More f 2,000 lx but could grow at the periodic illumination. The specific growth rate of M. viridis was lower than those of other species of Microcystis. These results suggested that M. viridis was more difficult to dominate than other Microcystis in summer.Moreover, we studied the effect of heavy metals on the growth of Microcystis spesies. Toxicity of copper, cadmium and cobalt on the growth of blue-green algae M. aeruginosa K3A, M. viridis, Anabaena affinis and green algae Chlorella ellipsoidea was determined in the liquid medium. The EC_<50> (effective concentration, 50% inhibition of the specific growth rate of control) of copper for M. aeruginosa K3A, M. viridis, A. arrinis, C. ellipsoidea were 0.019muM, 0.023muM, 0.018muM and 0.58muM, respectively, Copper showed the severest toxicity to the growth of these glgae compared with cadmium and cobalt. The sensitivity to copper of three species of blue-green algae was 30 times higher than that of the green algae C. llipsoidea. Chelating agents such as EDTA and EGTA showed the stroug reducing effect on the copper toxicity. Copper concentratin in the surface water of Lake Kasumigaura was 0.017muM, which was the almost same to the value of EC_<50> for blue-green algae. However, the EC_<50> of copper for M. aeruginosa K3A in the lake water was found to be 3 times larger than in the liquid medium. From these facts, it is assumed that natural ligands in the lakewater stimulated the growth of blue-green algae to make algal bloom. Less

本文对绿色微囊藻的水华进行了野外和室内实验研究。在1988-1990年的夏秋季进行了一项野外调查，以弄清鹿儿湖微囊藻优势种的变化。夏季优势种为铜绿假单胞菌和水华分枝杆菌，秋季优势种为绿色分枝杆菌。铜绿假单胞菌的现存量与水温有较高的相关性，而绿色微囊藻和韦氏微囊藻的现存量与水温无相关性。采用分批培养的方法研究了温度和光照对绿色分枝杆菌N-1和NIES 102的比生长速率和产藻量的影响。绿色分枝杆菌N-1和绿色分枝杆菌NIES 102在25℃和30℃时的比生长速率最大。绿色微囊藻的生长温度低于其他微囊藻。绿色木霉N-1在…的连续光照下不能生长2,000lx以上，但在周期性光照下可以生长。绿色微囊藻的比生长率低于其他微囊藻。这些结果表明，在夏季，绿色微囊藻比其他微囊藻更难占优势。此外，我们还研究了重金属对微囊藻生长的影响。在液体介质中测定了铜、镉、钴对铜绿微囊藻K3A、绿色微囊藻M、近缘鱼腥藻和小球藻椭圆形小球藻的生长毒性。铜对铜绿微囊藻K3A、绿色微囊藻、绿微囊藻、椭圆微囊藻的EC_(50)分别为0.019µM、0.023µM、0.018µM和0.58µM，表现出比镉和钴更强的毒性。三种蓝藻对铜的敏感性是绿藻C.llipsoda.的30倍。EDTA和EGTA等络合剂对铜毒有明显的减毒作用。鹿儿湖表层水中铜的浓度为0.017微米，与蓝藻的EC50和Gt；值基本一致。然而，铜在湖水中对铜绿假单胞菌K3A的EC_(50)和EC_(50)却是在液体介质中的3倍。根据这些事实，推测湖水中的天然配体刺激了蓝藻的生长，使藻类大量繁殖。较少