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The Study of ExtraCellur Matrix and Cytoskelton related with Epithelial Mesenchymal Interaction during the Organogensis

器官发生过程中细胞外基质和细胞骨架与上皮间质相互作用的研究

基本信息

批准号：
03660313
负责人：
AMASAKI Hajime
金额：
$ 1.28万
依托单位：
Nippon Veterinary and Animal Science University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660313/
关键词：
Organogenesis Interaction ECM Cytoskelton Mesenchyme Epithelium interaction Mesencyme

项目摘要

Present study reveals that organogenesis of bovine ruminal papaillae and palatine ridge, both of which are developed under the controle of the epithelial-mesenchymal interaction, may be regulated by some new type proteoglycan (PGs) molecules. Since these PGs expressed spatially and temporally during the specific developmental periods of the bovine ruminal papaillae and palatine ridge (in preparation of submission).Cell-adhesion molecules such as integlin and cytoskeleton molecule such as cytokeratines are also examined for the signalin peptides or the functional markers of the developmental epithelial cells (in preparetion of submission). We specially focased on beta_1, alpha_3, alpha_4, alpha_5, alpha_v integline subunits which are known to contact with the "RGD" sequences in the cell-adhesion site of the fibronectine molecule. Our results showed that beta_1 integline subunit was most intensely related with the organogenetic process of bovine ruminal papaillae and palatine ridge, and alpha_3 and alpha_5 subunits were slightly. It was also revealed that the expressions of some cytokeratine molecules might be correlated with the epithelial differentiation of these developmental systems.Further morre valuable results may be expected for the molecular biological studies, if there would be more times and any grant-in-aid.

本研究表明，在上皮-间质相互作用的调控下，牛瘤胃乳头和腭嵴的器官发生可能受到一些新型蛋白多糖（PGs）分子的调控。由于这些PG在牛瘤胃乳突和腭嵴的特定发育时期在空间和时间上表达（在提交的准备中），因此还检测了细胞粘附分子如整合素和细胞骨架分子如细胞角蛋白的信号肽或发育上皮细胞的功能标记（在提交的准备中）。我们特别关注了与纤维连接蛋白分子的细胞粘附位点上的“RGD”序列相接触的β_1、α_3、α_4、α_5、α_v整合素亚基。结果表明，整合素β_1亚基与牛瘤胃乳头和腭嵴的器官发生过程关系最密切，α_3和α_5亚基与此关系不大。本研究还揭示了某些细胞角蛋白分子的表达可能与这些发育系统的上皮分化有关，如能有更多的时间和资助，将为分子生物学研究提供更多有价值的结果。