Evaluation of calf viability based the concentrations of oestrone sulphate in pregnant animals

用酶免疫分析法(EIA)测定了妊娠动物血浆中主要的胎盘激素硫酸雌酮(OS)的浓度，以监测怀孕动物的妊娠状态。同时测定尿液OS、乳汁OS和粪便OS。怀孕期间的样本每月从母牛和母马身上收集。EIA方法采用高结合能力免疫板和小鼠OS单抗的抗原包被试验。用尿肌酸调节尿液稀释度，妊娠奶牛140d后血浆中OS浓度升高超过1.1 ng/ml，显著高于非妊娠奶牛(0.36 ng/ml)。妊娠奶牛第100天乳汁中OS含量为236pg/ml，显著高于非妊娠奶牛的14pg/ml。粪便中OS浓度与血浆中OS浓度呈显著正相关。未孕母马尿中OS浓度为0.16μg/mg cre，妊娠2个月时为0.46μg/mg cre。非妊娠母马血浆中OS浓度为19.7~31.5 ng/ml，乳汁、尿液和粪便中OS浓度均为非侵入性采样，妊娠2个月后OS浓度显著高于未妊娠母牛和母马。

Plasma concentrations of oestrone sulphate (OS), which is a major placental hormone were measured using an enzyme immunoassay (EIA) to monitor pregnancy status in pregnant animals. Concurrently, urine OS, milk OS and fecal OS were measured. Samples during pregnancy were collected monthly from cows and mares. EIA protocol was antigen coated assay using high binding capacity immunoplate and mouse monoclonal antibody for OS. Urine creatine was used for adjusting urine dilution.OS concentrations in plasma samples increased more than 1.1 ng/ml after d 140 in pregnant cows, which was significantly higher than those in non-pregnant cows (0.36 ng/ml). OS concentrations in milk showed 236 pg/ml after d 100 in pregnant cows, which was also significantly higher than those in non-pregnant cows (14 pg/ml). Os concentrations in fecal samples showed significant correlation with those in plasma samples. OS concentrations in urine samples ranged from 0.16 μg/mg cre in non-pregnant mares to 0.46 μg/mg cre in second month pregnancy. OS concentrations in plasma samples ranged from 19.7 ng/ml in non-pregnant to 31.5 ng/ml in first month of pregnant mares.OS concentrations in milk, urine and feces determined by EIA were non-invasive sampling, and showed significantly higher levels after second month pregnancy compared to those in non-pregnant cows and mares.