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Evaluation of calf viability based the concentrations of oestrone sulphate in pregnant animals

根据怀孕动物硫酸雌酮的浓度评估犊牛活力

基本信息

批准号：
13660320
负责人：
HAMANA Katsumi
金额：
$ 1.6万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660320/
关键词：
Oestrone Sulphate EIA Pregnancy monitoring Plasma oestrone sulphate Milk oestrone sulphate Urine oestrone sulphate Fecal oestrone sulphate エストロンサルフェート濃度乳汁中OS濃度血液中OS濃度胎子の成熟度

项目摘要

Plasma concentrations of oestrone sulphate (OS), which is a major placental hormone were measured using an enzyme immunoassay (EIA) to monitor pregnancy status in pregnant animals. Concurrently, urine OS, milk OS and fecal OS were measured. Samples during pregnancy were collected monthly from cows and mares. EIA protocol was antigen coated assay using high binding capacity immunoplate and mouse monoclonal antibody for OS. Urine creatine was used for adjusting urine dilution.OS concentrations in plasma samples increased more than 1.1 ng/ml after d 140 in pregnant cows, which was significantly higher than those in non-pregnant cows (0.36 ng/ml). OS concentrations in milk showed 236 pg/ml after d 100 in pregnant cows, which was also significantly higher than those in non-pregnant cows (14 pg/ml). Os concentrations in fecal samples showed significant correlation with those in plasma samples. OS concentrations in urine samples ranged from 0.16 μg/mg cre in non-pregnant mares to 0.46 μg/mg cre in second month pregnancy. OS concentrations in plasma samples ranged from 19.7 ng/ml in non-pregnant to 31.5 ng/ml in first month of pregnant mares.OS concentrations in milk, urine and feces determined by EIA were non-invasive sampling, and showed significantly higher levels after second month pregnancy compared to those in non-pregnant cows and mares.

采用酶免疫测定法（EIA）测定了妊娠动物血浆中主要胎盘激素硫酸雌酮（OS）的浓度，以监测妊娠状态。同时测定尿液OS、乳汁OS和粪便OS。妊娠期间每月从奶牛和奶牛中采集样本。EIA采用高结合力免疫板抗原包被法和小鼠抗OS单克隆抗体，以尿肌酸调节尿稀释度，妊娠母牛140 d后血浆中OS浓度增加1.1ng/ml以上，显著高于非妊娠母牛（0.36ng/ml）。妊娠母牛在第100天后乳汁中的OS浓度为236 pg/ml，也显著高于非妊娠母牛（14 pg/ml）。粪样中Os浓度与血浆中Os浓度呈显著相关。未孕妇女尿中OS浓度为0.16 μg/mgcre，孕2个月尿中OS浓度为0.46 μg/mgcre。血浆中OS浓度范围为19.7 ng/ml（未妊娠）~ 31.5 ng/ml（妊娠1个月）。EIA法测定的牛奶、尿液和粪便中OS浓度为非侵入性采样，妊娠2个月后显著高于未妊娠和妊娠1个月的水平。