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Epidemiological, hereditary, morphological and virological analysis in bovine congenital defects.

牛先天缺陷的流行病学、遗传性、形态学和病毒学分析。

基本信息

批准号：
15380216
负责人：
HAMANA Katsumi
金额：
$ 4.35万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

In 2000-2005, 535 calves with congenital defects were collected in Kagoshima. Epidemiological, clinical, pathological and virological investigations clarified the outbreaks of Akabane disease characterized by hydranencephaly and arythrogryposis in early 2000, Chuzan disease characterized by hydranencephaly and cerebellar hypoplasia in early 2002 and Aino virus infection characterized by micrencephaly and cerebellar hypoplasia in early 2003. Thereafter, there are no big outbreaks of the defects by these arbovirus infection.Single-tube multiplex reverse transcription-polymerase chain reaction (mRT-PCR) assay was developed to detect and identify arboviruses in infected cell-culture fluids and field specimens. This technique was sensitive and reliable to detect the Chuzan, Ibaraki, Bluetongue, Akabane and Peaton viruses. Aino virus was inoculated directly to the fetus in utero. Newborn calves had the congenital abnormalities including arthrogryposis, hydranencephaly and cerebellar hypoplasia. This technique first demonstated Aino virus as a causative agent of the defects.In the morphological investigations of cardiovascular system, conjoind heart, premature closure of the foramen ovale, partial anomalous pulmonary venous connection and atrioventricular septal defects were observed and reported.In the series of this research projects, the book titled ‘Color Atlas of Bovine Congenital Defects' was published. In this book, the most of the defects found in Japan is explained with the clear 763 color photographs. This is the first book about the bovine congenital defects in Japan and will be very useful for research workers, veterinarians and animal producers.

2000年至2005年，在鹿儿岛收集了535头有先天性缺陷的小牛。流行病学、临床、病理和病毒学调查表明，2000年初爆发了以无水脑畸形和甲状腺增生为特征的阿卡巴纳病，2002年初爆发了以无水脑畸形和小脑发育不全为特征的Chuzan病，2003年初爆发了以小脑畸形和小脑发育不全为特征的Aino病毒感染。此后，这些虫媒病毒感染的缺陷没有发生大的暴发。建立了单管多重逆转录聚合酶链反应（mRT-PCR）检测和鉴定感染细胞培养液和野外标本中的虫媒病毒的方法。该方法对楚山病毒、茨城病毒、蓝舌病毒、赤叶病毒和皮顿病毒检测灵敏可靠。胎儿在子宫内直接接种禽流感病毒。新生牛犊有先天性畸形，包括关节挛缩、无水脑畸形和小脑发育不全。该技术首次证明了aio病毒是缺陷的致病因子。在心血管系统形态学检查中，观察并报道了联合心脏、卵圆孔过早闭合、部分肺静脉连接异常和房室间隔缺损。在该系列研究项目中，出版了《牛先天性缺陷彩色图集》一书。在这本书中，用清晰的763彩色照片解释了在日本发现的大部分缺陷。这是第一本关于日本牛先天性缺陷的书，将对研究工作者、兽医和动物生产者非常有用。