DIRECT ULTRAVIOLET IRRADIATION OF FETAL PANCREAS ZENOGRAFTS FOR REVERSAL OF THE EXPERIMENTAL DIABETIC DOGS

直接紫外线照射胎胰异种移植物逆转实验犬糖尿病

• 批准号: 03660322
• 负责人: TAURA Yasuho
• 金额: $ 0.45万
• 依托单位: YAMAGUCHI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660322/
• 关键词: FETAL PANCREAS; PANCEAS TRANSPLANTATION; XENOGRAFT; ULTRAVIOLET IRRADIATION; DIABETES; DOG; IMMUNOALTERATION; 異種移植; ドナ-処理

THE PURPOSE OF THIS STUDY IS TO EXAMINE WHETHER FETAL PANCREATIC XENO-GRAFTS CAN EXERT THEIR EFFECT ON THE DIABETIC RECIPIENTS TO USING BY DIRECT ULTRAVIOLET (UV)-IRRADIATION OF THE DONORS IN VIVO AND TO EXAMINE BY THE MIXED ISLETS AND LYMPHOCYTE CULTURE(MILC)IN VITRO. [MATERIALS AND METHODS] ELEVEN TOTAL PANCREATECTOMIED MONGREL DOGS WERE USED TO THE RECIPIENTS OF UV-TREATED FETAL PANCREAS TRANSPLANTATION OR TO THE CONTROL GROUP.THE FETAL PANCREAS TISSUES WERE ENGRAFTED INTO OMENTUM POUCH OR SPLEEN AFTER DIRECT UV-IRRADIATION(UV LIGHT: UVM-57,302NM, UVP INC.,IRRADIATION TIME: 10MIN). [RESULTS] DIRECT UV-IRRADIATION OF FETAL PANCREAS PREVENTED THEM FROM INITIATING PROLIFERATIVE RESPONSES IN XENO-GENEIC MILC.THE RESPONSE REDUCED TO 73.4*8.8%(MEAN*SE)WITH GOOD VIA-BILITY COMPARED WITH NO UV-IRRADIATION.AT THE SURVIVAL DAYS FROM TOTAL PANCREATECTOMY WERE 84.2*14.1 DAYS IN XENOGRAFT.THE OTHER SIDE, THEY WERE SURVIVED AT 24.6*6.5 DAYS AND MORE THAN 252.2(] SY.-+.[)66.1 DAYS,IN NON-TRANSPLANTATION AND ALLOGRAFT RESPECTIVELY.DAILY INSULIN REQUIREMENTS, URINE GLUCOSE LEVELS WERE TRENDED TO DECREASE AFTER ZENO-TRANSPLANTATION. [CONCLUSION] THE ALTERATIONS IN IMMUNE RESPONSIVENESS RESULTING FROM THE USE OF UV-TREATED XENOGENEIC FETAL PANCREAS MAY ALLOW LONG-TERM GRAFT SURVIVAL WITHOUT REQUIRING LARGE DOSES OF IMMUNOSUPPRESSIVE DRUGS.

本研究的目的是通过体内供体直接紫外线照射和体外胰岛和淋巴细胞混合培养来观察胎儿胰腺异种移植物是否能对糖尿病受体发挥作用。【材料与方法】将11只全胰切除的杂种犬作为紫外线处理的胎儿胰腺移植受者或对照组。直接紫外线照射（紫外线：uvm-57,302nm, uvp inc .，照射时间：10min）后，将胎儿胰腺组织植入网膜袋或脾脏。[结果]胎儿胰腺的直接紫外线照射阻止了它们在异种乳汁中启动增殖反应。与无紫外线照射相比，响应降低到73.4*8.8%（平均* se），透光性良好。全胰切除术后的生存天数为84.2天*异种移植的生存天数为14.1天。非移植组和同种异体移植组存活时间分别为24.6*6.5天和252.2(]y .-+.[)66.1天以上。肾移植后每日胰岛素需用量、尿糖水平均有下降趋势。[结论]使用紫外线处理的异种胎儿胰腺引起的免疫反应性改变可能使移植物长期存活，而不需要大剂量的免疫抑制药物。

项目成果

TAURA,Y.,YASUDA,M.,UNE,S.,TAKASHIMA,MIKASA,N.,AND NAKAMA,S.: "DOSE IT IMPROVE DIABETES BY XENOGRAFTS USING ULTRAVIOLET IRRADIATED FETAL PANCREAS?(IN JAPANESE)" ANNUAL MEETING OF ANIMAL CLINACAL RESEARCH FOUNDATION,PROCEEDINGS. 13. 49-54 (1992)

TAURA,Y.、YASUDA,M.、UNE,S.、TAKASHIMA、MIKASA,N. 和 NAKAMA,S.：“使用紫外线照射胎儿胰腺进行异种移植可以改善糖尿病吗？（日语）”动物年会

宇根 智,田浦 保穂,宮本 忠,江島 博康,中間 實徳: "犬の異系間輸血による免疫応答の変化" 小動物臨床研究会年次大会proceedings. 12. 430-436 (1991)

Satoshi Une、Yasuho Taura、Tadashi Miyamoto、Hiroyasu Ejima 和 Minoru Nakama：“狗跨系统输血导致的免疫反应变化”小动物临床研究学会年会论文集 12. 430-436 (1991)。

Taura,Y., Stein,E. and Mullen,Y.: "Suppression of MLR and DTH responses in miniature swine by pretreatment with ultra violet(UV)-irradiated peripheral blood lymphocytes." Jpn. J. Vet. Sci.52. 1163-1170 (1990)

陶拉，Y.，斯坦，E.

UNE,S.,TAURA,Y.,NAKAMA,S.,AND EJIMA,H.: "IMMUNOSUPPRESSION IN DOGS BY PRETRANSFUSIONS WITH ULTRAVIO-LET(UV)-IRRADIATED WHOLE BLOOD." J.VET.MED.SCI. 53-5. 811-816 (1991)

UNE,S.、TAURA,Y.、NAKAMA,S. 和 EJIMA,H.：“通过预先输注紫外线照射的全血对狗进行免疫抑制。”

MULLEN,Y.,TAURA,NAGATA,M.,MIYAZAWA,K.,AND STEIN,E.: "SWINE AS MODELS IN BIOMEDICAL RESEARCH(1ST EDI.).2.SWINE AS MODEL FOR PANCREATIC BETA-CELL TRANSPLANTATION." SWINDLE,M.M.(EDI.)IOWA STATE UNIV.PRESS,AMES. 16-34 (1992)

MULLEN,Y.,TAURA,NAGATA,M.,MIYAZAWA,K.,and STEIN,E.：“猪作为生物医学研究的模型（第 1 版）。2.猪作为胰腺 β 细胞移植的模型。”