Investigation of the mechanism of intervertebral

椎间融合机制研究

• 批准号: 10839010
• 负责人: TAURA Yasuho
• 金额: $ 2.11万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10839010/
• 关键词: Dog; Intervertebral disc herniation; Nucleus pulposus; Chondrodystrophoid; Non-chondrodystrophoid; Cathepsin L; MRI; Cystein proteinases; システインプロティナーゼ

In this research, we investigated the cause of degeneration intervertebral disc (IVD) and the mechanism of IVD herniation in dogs by means of magnetic resonance imaging (MRI) in Yamaguchi University Hospita. We divided two study groups in which were chondrodystrophoid (CHD) breed, such as dachshund or beagle, and non-chondrodystrophoid (NCHD) breed, such as maltese. In IVD of CHD breed, the signal intensity of T2-weighted image was higher than that of NCHD. This was suggested that there were more moistly in IVD of CHD than that of NCHD because there were chondrocyte in the nucleus pulposus in CHD and not in NCHD. Then we investigated the biochemical activity of several cystein proteinases in the nucleus pulposus (NP), which was center of IVD, and anulus fibrosus (AF), which was surround of NP, by means of biochemical analysis. In IVD of beagle dog which was CHD group, there were much more cathepsin L than cathepsin B, which both proteinase were a member of cystein proteinase family. And proteinase activity was higher at NP than AF in each proteinases. These results suggested that the mechanism of IVD degeneration and/or herniation was related to region specificity or pH specificity because of cathepsin L activity was higher at acidic region. This may be important key to investigate the cause of IVD degenetation and herniation in dogs.

在本研究中，我们调查的原因退变椎间盘（IVD）和IVD突出的机制，通过磁共振成像（MRI）在山口大学医院的狗。我们分为两个研究组，其中软骨营养不良（CHD）品种，如腊肠犬或比格犬，和非软骨营养不良（NCHD）品种，如马耳他。CHD犬IVD在T2加权像上的信号强度高于NCHD犬。提示CHD髓核内有软骨细胞存在，而NCHD髓核内无软骨细胞存在，是CHD髓核内软骨细胞增多的原因。然后，我们采用生化分析方法，对位于椎间盘中心的髓核（NP）及其周围的纤维环（AF）中几种半胱氨酸蛋白酶的生化活性进行了研究。CHD组Beagle犬IVD中组织蛋白酶L的含量明显高于组织蛋白酶B，两者均属于半胱氨酸蛋白酶家族。各蛋白酶的蛋白酶活性NP高于AF。结果提示，组织蛋白酶L在酸性区活性较高，导致IVD变性和/或疝出的机制与区域特异性或pH特异性有关。这可能是研究犬IVD变性和疝形成原因的重要关键。

项目成果

Taga,A,Taura,Y: "Magnetic resonance imaging of syringomyelia in fivu dogs"J.Small Animal Practice. 41(accepted). (2000)

Taga,A,Taura,Y：“fivu 狗脊髓空洞症的磁共振成像”J.小动物实践。

Taga,A.・Taura,Y.: "The advantage of magnetic resonanoe imaging in diagnosis of cauda equina syndrome in dogs" J.Vet.Med.Sci.60(12). 1345-1348 (1998)

Taga, A.·Taura, Y.：“磁共振成像在狗马尾综合征诊断中的优势”J.Vet.Med.Sci.60(12) (1998)。

Sanenori NAKAMA: "Spinal diseases in small animals : Diagnosis and treatment"Inter Zoo. 211 (1998)

Sanenori NAKAMA：“小动物脊柱疾病：诊断和治疗”Inter Zoo。

田賀淳夫・田浦保穂: "犬の嚢腫性二分脊椎症例に対する背側椎弓切除術および皮膚洞切除術" 日獣会誌. 51(10). 591-594 (1998)

Atsuo Taga 和 Yasuho Taura：“犬囊性脊柱裂的背侧椎板切除术和皮肤窦切除术”，日本兽医学会杂志 51(10) (1998)。

中市統三: "小動物の脊椎・椎間板疾患:診断と治療(中間實徳監修)" インターズー, 211 (1998)

Sozo Nakaichi：“小动物脊柱和椎间盘疾病：诊断和治疗（由 Minoru Nakaichi 监督）” Interzoo，211（1998）