A NEW CRYOPRESERVATION OF PANCREATIC ISLETS FOR CURE DIABETES IN VETERINARY MEDICINE

这次研究的目的是采取更大的一部分是下一件事。通过对动物胰岛的体外培养，包括胰岛的分离和保存，寻找能够耐受实际移植的胰岛的保存方法。之后，通过使用冷冻和融合的胰岛将其移植到实验性糖尿病模型动物中，并在体内检查效果。此外，使由于胰岛冷冻引起的胰岛素分泌能力下降的原因变得明显。冷冻装置使用山口大学兽医外科部门拥有的程序冷冻机（MPF - 1000，EYELA）和液氮。它从大鼠、猪或牛身上获得胰岛模型，猪从肉的中心获得胰岛。分离培养操作使用同一泌尿外科的洁净工作台和冷却离心机。 ...更多信息 n.糖尿病模型动物可维持全胰切除或按Tauras方法注射链脲佐菌素所造成的糖尿病状态，并趋于稳定。从现在开始，胰岛将被冷冻，融合后的胰岛将被移植。用体外观察法，测定胰岛分泌胰岛素能力的发挥，并对胰岛进行形态学指标和功能学指标的检测。在体内，通过各项监测和移植结算，最终再次提取确认受者是否糖尿病状态的计划，并进行组织检查，使受者再次成为糖尿病状态，胰腺胰岛的冷冻是在二甲基亚砜（DMSO）存在下进行的，而之前是在乙二醇（EG）存在下进行的。存在这也是世界上第一次证明即使是EG也能保持胰岛的冷冻。此外，EG明确指出，与DMSO相比，浸泡时间和冷冻中的渗透压休克对胰岛的细胞毒性较低，并且冷冻更容易保存。少

The purpose of this research of taking the greater part is the next thing. The way of keeping the pancreatic islets which can put up with an actual transplant is looked up by using the animal's pancreatic islets with in vitro including the pancreatic islets separate and preservation. After that, it is transplanted to the experimental diabetes model animal by using the pancreatic islets which freeze and which fused, and the effect is examined with in vivo. Furthermore, make the cause of the decline of the insuin secretion ability play due to freezing of the pancreatic islets distinct.A freezing device used the program freezer (MPF - 1000, EYELA) that a Yamaguchi University Department of Veterinary Surgery had, and liquid nitrogen. It got a pancreatic islets model from the rat, the pig which obtaines it from the center of the meat, or the cattle as well. The clean bench of the same Department of Vaterinary Surgery and a cooling centrifuge were used for the separation cultivation operatio … More n. A diabetes model animal could maintain the diabetes condition that it was made with the all pancreases extraction or the streptozotocin injection in accor dance with Tauras' method and which became stable. The pancreatic islets which will freeze from now on in them and which fused will betransplanted. It was observed with in vitro, the insulin secretion ability play of the pancr eatic islets was measured, and a pancreatic islets was examined in the form learning target and the function study target. With in vivo, the plan of confirming whether the diabetes condition of the recipient is monitored by each item and transplant settlement is finally extracted again and organization inspection is carried out and a recipient becomes diabetes condition again, it is progressing.Freezing of the pancr eatic islets being done if it could freeze only under the existence of dimethyl sulfoxide (DMSO) until before was done under the ethylen glycol (EG) existence. It could be proved for the first time that even EG could keep freezing of the pancreatic island in the world as a result. Moreover, EG made it clear that it was proved that cell toxicity to the pancreatic islets by soaking head time and an osmotic shock in freezing were low in comparison with DMSO and freezing could be kept more easily. Less