Application of autotransfusion with -80℃ canine erythrocyte cryopreservation in veterinary medicine

-80℃犬红细胞冻存自体输血在兽医中的应用

• 批准号: 16580262
• 负责人: TAURA Yasuho
• 金额: $ 2.43万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580262/
• 关键词: canine; -80℃; autotransfusion; hydroxyethyl starch (HES); erythrocyte; cryopreservation; veterinary medicine; cryoprotectant

In this canine erythrocytes study, the effect of -196℃ or two step method(-196℃→80℃) cryopreservation was examined. Erythrocytes were mixed with cryoprotective solution containing hydroxyethyl starch (HES) or glycerol (GL). This mixture was filled into plastic bag made of vinyl chloride and frozen to -196℃ in liquid nitrogen. It was transferred to -80℃ deepfreezer and was preserved for 3 month. After thawing, erythrocytes were not washed, and autotransfused.1)The canine erythrocytes frozen HES and GL at -196℃ could be autotransfused without side effects. The HES was considered to have a practical value rather than the GL.2)HES was used a cryoprotective solution, the possibility of the plentiful cryopreservation of canine erythrocytes at two step method (-196℃→-80℃) was found out.3)Canine erythrocytes were cryopreserved at -80℃ with HES for three months or more was found out. In recovery rate, osmotic fragility test and scanning electron microscopy, significant difference was not observed in each period. Therefore, -80℃ cryopreservation can preserve canine erythrocytes longer than liquefied preservation.4)The canine erythrocytes frozen HES at two step method could be autotransfused without side effects.This two step method that could be autotransfused, and was considered to have a practical value in veterinary medicine.

本研究对犬红细胞进行了-196℃和两步法（-196℃→80℃）冻存效果的研究。将红细胞与含有羟乙基淀粉（HES）或甘油（GL）的冷冻保护液混合。将该混合物装入氯乙烯塑料袋中，并在液氮中冷冻至-196℃。将其转移至-80℃深冻箱中并保存3个月。1）-196℃冻存的犬红细胞HES和GL均能进行自体回输，且无副作用。结果表明，羟乙基淀粉（HES）比GL更具有实用价值; 2）以羟乙基淀粉（HES）为冻存液，探索了两步法（-196℃→-80℃）冻存犬红细胞的可能性; 3）以羟乙基淀粉（HES）为冻存液，探索了-80℃冻存犬红细胞3个月以上的可行性。在复苏率、渗透脆性试验和扫描电镜检查方面，各时期差异不显著。因此，-80℃冷冻保存的犬红细胞比液化保存的犬红细胞保存时间更长。4）两步法冻存的犬红细胞可进行自体回输，且无副作用，两步法可进行自体回输，在兽医学上具有实用价值。

项目成果

A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation.

甘油与羟乙基淀粉对犬红细胞冻存影响的比较研究。

• 发表时间: 2004
• 期刊: Journal Veterinary Medical Science Vol.66
• 作者: Kim;H.;Tanaka;S.;Une;S.;Nakaichi;M.;Sumida;S.;Taura;Y.
• 通讯作者: Y.

A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopredervation.

甘油和羟乙基淀粉在犬红细胞冷冻保存中作用的比较研究。

• 发表时间: 2004
• 期刊: J.Vet.Med.Sci 66.12
• 作者: Kim;H.;Tanaka;S.;Une;S.;Nakaichi;M.;Taura;Y.
• 通讯作者: Y.

Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch.

磷酸烯醇式丙酮酸在羟乙基淀粉犬红细胞冻存中的应用

• 发表时间: 2005
• 期刊: Cryo Letters 26・1
• 作者: Kim;H;et al.
• 通讯作者: et al.

A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation.Dec;(2004)

甘油和羟乙基淀粉对犬红细胞冻存效果的比较研究。Dec;(2004)

• 发表时间: 2004
• 期刊: J Vet Med Sci 66・12
• 作者: Kim;H.et al.
• 通讯作者: H.et al.

Incubation of canine red blood cells before freezing leads to an improuved saline stability and morphology after thawing when using a hydroxyethyl starch cryopresevation protocol.

当使用羟乙基淀粉冷冻保存方案时，冷冻前孵育犬红细胞可改善解冻后的盐水稳定性和形态。

• 发表时间: 2005
• 期刊: Low Temp.Med 31
• 作者: Kim;H;et al.
• 通讯作者: et al.