Structure and function of chemotactic factor, C5a, and its receptor

趋化因子C5a及其受体的结构和功能

• 批准号: 03670211
• 负责人: TAKEDA Junji
• 金额: $ 1.22万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670211/
• 关键词: Complement; C5a; Chemotactic activity; Monoclonal antibody; Epitope; 補体活性化フラグメントC5a; モノクロ-ナル抗体; エピト-プマッピッグ

C5a is a potent chemotactic peptide derived from complement C5. C5a is immediately inactivated to C5a des Arg by carboxypeptidase N after activation of complement. Physiological C5a action occur through C5a receptor on phagocytic cells. In this study, we determined epitopes on C5a to understand the role of C5a using series of anti C5a monoclonal antibodies and N-terminal or C-terminal oligopeptides from C5a. Bindings between C5a des Arg and anti C5a monoclonal antibody were not inhibited by either N-terminal or C-terminal oligopeptides. However C-terminal oligopeptides without Arg could inhibit binding between C5a des Arg and some anti C5a monoclonal antibodies. These results demonstrate that some anti C5a monoclonal antibodies can recognize C-terminal peptide only without Arg.

C5a是一种有效的趋化肽，来源于补体C5。补体激活后，C5a立即被羧肽酶N灭活为C5a des Arg。C5a的生理作用是通过C5a受体作用于吞噬细胞。在本研究中，我们利用一系列抗C5a单克隆抗体和来自C5a的n端或c端寡肽，确定了C5a的表位，以了解C5a的作用。C5a des Arg与抗C5a单克隆抗体的结合不受n端或c端寡肽的抑制。不含精氨酸的c端寡肽可抑制C5a des精氨酸与部分抗C5a单克隆抗体的结合。这些结果表明，一些抗C5a单克隆抗体只能识别c端肽，而不识别精氨酸。

项目成果

Y.C.Kim: "Covalent Binding of C3b to C4b within the classical complement pathway C5 convertase" J.Biol.Chem.267. 4171-4176 (1992)

Y.C.Kim：“经典补体途径 C5 转化酶内 C3b 与 C4b 的共价结合”J.Biol.Chem.267。

Kim,Y-U.: "Covalent binding of C3b to C4b withen the classical complement pathway C5 convertase:Determination of amino acid residues involved in ester linkage formation." J.Biol,Chem.267. 4171-4176 (1992)

Kim,Y-U.：“经典补体途径 C5 转化酶中 C3b 与 C4b 的共价结合：确定参与酯键形成的氨基酸残基。”

Pramoonjago,P.: "Bactericidal activity of C9-deficient human serum." J.Immunol.148. 837-843 (1992)

Pramoonjago,P.：“C9 缺陷型人血清的杀菌活性。”

Pramoonjago, P: "Bactericidal activity of C9-deficient human serum" J. Immunol. 148. 837-843 (1992)

Pramoonjago, P：“C9 缺陷型人血清的杀菌活性”J.Immunol。

酒井 寛: "補体アナフィラトキシンC5aのエピト-プ解析" 日本細菌学雑誌. 47. 120 (1992)

Hiroshi Sakai：“补体过敏毒素 C5a 的表位分析”日本细菌学杂志 47. 120 (1992)。