Physiological roles of epidermal sphingolipids with conditional gene targeting.

条件基因靶向表皮鞘脂的生理作用。

• 批准号: 16390316
• 负责人: TAKEDA Junji
• 金额: $ 9.15万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390316/
• 关键词: sphingolipid; epidermis; SPT; conditional gene targeting; barrier function; mouse; 遺伝子破壊; Arnt; コンディショナルジーンターゲティング; desaturase

Although many scientists predict sphingolipids play important roles in epidermis, its direct evidence is still lacking. To investigate physiological roles of epidermal sphingolipids, we generated a mouse line lacking the first step gene of sphigolipid biosynthesis, SPT, in epidermis. The SPT deficient mice died within 3 weeks after birth. Barrier functions were impaired in the SPT deficient mice. With mass spectrometry analysis sphingolipids bearing three fatty acids were abnormally elevated. These results indicate that de novo synthesis of sphingolipids is important for maintenance of epidermal barrier functions.

尽管许多科学家预测鞘脂在表皮中起重要作用，但其直接证据仍然缺乏。为了研究表皮鞘脂的生理作用，我们在小鼠表皮中培养了缺乏鞘脂生物合成第一步基因（SPT）的小鼠。SPT缺陷小鼠在出生后3周内死亡。SPT缺陷小鼠的屏障功能受损。质谱分析鞘脂含有三种脂肪酸异常升高。这些结果表明鞘脂从头合成对维持表皮屏障功能很重要。

项目成果

Target-site preferences of Sleeping Beauty transposons

• DOI: 10.1016/j.jmb.2004.09.086
• 发表时间: 2005-02-11
• 期刊: JOURNAL OF MOLECULAR BIOLOGY
• 影响因子: 5.6
• 作者: Liu, GY;Geurts, AM;Hackett, PB
• 通讯作者: Hackett, PB

Disruption of Stat3 reveals a critical role in both the initiation and promotion stages of epithelial carcinogenesis.

Stat3 的破坏揭示了上皮癌发生的起始和促进阶段的关键作用。

• 发表时间: 2004
• 期刊: J.Clin.Invest. 114
• 作者: Kouno;M.;Chan K.S
• 通讯作者: Chan K.S

Angiotensin-converting enzyme is a GPI-anchored protein releasing factor crucial for fertilization.

• DOI: 10.1038/nm1179
• 发表时间: 2005-03
• 期刊: Nature medicine
• 影响因子: 82.9
• 作者: Kondoh G;Tojo H;Nakatani Y;Komazawa N;Murata C;Yamagata K;Maeda Y;Kinoshita T;Okabe M;Taguchi R;Takeda J
• 通讯作者: Takeda J

Raf-1 regulates Rho signaling and cell migration.

RAF-1调节RHO信号传导和细胞迁移。

• DOI: 10.1083/jcb.200409162
• 发表时间: 2005-03-14
• 期刊: JOURNAL OF CELL BIOLOGY
• 影响因子: 7.8
• 作者: Ehrenreiter, Karin;Piazzolla, Daniela;Velamoor, Vanishree;Sobczak, Izabela;Small, J Victor;Takeda, Junji;Leung, Thomas;Baccarini, Manuela
• 通讯作者: Baccarini, Manuela

Ahnak/Desmoyokin is dispensable for proliferation, differentiation and maintenance of integrity in mouse epidermis

• DOI: 10.1111/j.0022-202x.2004.23412.x
• 发表时间: 2004-10-01
• 期刊: JOURNAL OF INVESTIGATIVE DERMATOLOGY
• 影响因子: 6.5
• 作者: Kouno, M;Kondoh, G;Hashimoto, T
• 通讯作者: Hashimoto, T