ELECTRIC FIELDS ON METABOLISM OF A CHRONIC WOUND MODEL

在康复医学治疗的患者中， 褥疮和腿部溃疡。 这些疾病的发病率、死亡率 并且通常对常规治疗有抵抗力。 的疗效 电疗法在几十年的使用后仍不稳定， 仍然是伤口内的天然电源（即，的 “皮肤电池”）可以促进愈合。 我们建议详细说明成纤维细胞减少蛋白质的观察结果， 如果暴露于与内源性场相当的电场，则可以合成。 为此目的，将人真皮成纤维细胞在I型胶原真皮中培养， "等效"基质（DEM）暴露于离子电流。 初始 从我们的DEM模型的结果是有希望的。 他们认为， 扩散到20 mV/m，10 Hz的电场。（p，01）。 (Most经常 合成和增殖是相互的）。 采用相同的模型，我们提出了一个全面的研究， DEM模型对这些电场的生化反应。 我们计划 DNA、蛋白聚糖、胶原蛋白、纤连蛋白和 胶原酶 识别最易受电气影响的过程 影响，我们将调查响应的幅度和频率内 内源范围 如果皮肤电池对伤口愈合很重要， 细胞应该在1 - 10 Hz最敏感（即，低至5 - 20 m/m）。 创伤后早期，成纤维细胞经历纤维增生，并合成成纤维细胞。 蛋白聚糖和纤连蛋白。 后来，胶原蛋白的合成和 新生血管形成高峰。 我们将研究 刺激后0至14天，观察DEM中的成纤维细胞 生物合成转化发生在体外。 如果他们这样做，我们将有 令人信服的证据表明，皮肤电池有助于介导的反应， vivo. 最后，我们将研究早期的电效应。 细胞内事件，最终导致增殖和/或 合成.

Among the patients treated by Rehabilitation Medicine are those with decubitus and leg ulcers. These have significant morbidity, mortality and often are resistant to conventional treatment. The efficacy of electrotherapy is unsettled after decades of use and the possibility remains that a natural source of electricity within wounds(i.e., the "skin battery") may promote healing. We propose to elaborate the observation that fibroblasts decrease protein synthesis if exposed to electric fields comparable to endogenous fields. For this purpose, human dermal fibroblasts in type I collagen dermal "equivalent" matrices (DEM) are exposed to ionic currents. Initial results from our DEM model are promising. They suggests an increase in proliferation to electric fields of 20 mv/m, 10Hz.(p,01). (Most often synthesis and proliferation are reciprocal). Employing the same model, we propose a comprehensive study of the biochemical response of the DEM model to these electric fields. We plan radioisotope assays for DNA, proteoglycans, collagen, fibronectin and collagenase. Identifying the process most susceptible to electric effect, we will survey response to amplitudes and frequencies within the endogenous range. If the skin battery is important to wound healing, cells should be most sensitive at 1-10 Hz (i.e., down to 5-20 m/m). Early after wounding, fibroblasts undergo fibroplasia, and synthesis of proteoglycans and fibronectin. Later, collagen synthesis and neoangiogenisis peak. We will investigate the biochemical response of fibroblasts in DEM from 0 to 14 days after stimulation to see if these biosynthetic transformations occur in vitro. If they do, we will have compelling evidence that the skin battery help mediates the response in vivo. Lastly, we will investigate electric effects on early intracellular events that ultimately lead to proliferation and/or synthesis.