義歯床下における骨組織の動態に関する形態計測的検討

义齿基托下骨组织动力学的形态测量研究

• 批准号: 03670914
• 负责人: 佐藤 隆志
• 金额: $ 1.34万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670914/
• 关键词: 義歯床下組織; 骨組織の動態; 形態計測; 二重標識法

ラットの口蓋を対象として義歯床を装着し,義歯床下の非清掃状態の維持によって生じる剥離上皮細胞の停滞と遁出による持続的圧力の変化を義歯床下組織に加え,義歯床下における骨組織の吸収ならびに添加の動態に関して検討を行った.12週齢のウイスタ-系雄性ラット50匹の上顎臼歯部に接着したメタル・フレ-ムを介して義歯床を装着し,義歯床装着の1,2,3,4,5,6,7,8,9および10週後を観察期間とした.観察期間終了の9日前にテトラサイクリンを,2日前にカルセインを投与することによってラベリングを行った.得られた口蓋組織にVillanuevaの骨染色を施し,未脱灰研磨標本とし,申請の顕微X線撮影装置を用いた顕微X線写真の撮影後に,光学顕微鏡,蛍光顕微鏡および二次元画像解析装置を用いて観察と形態計測を行った.義歯床装置1週後では,骨の動態にはほとんど変化は認められなかった.義歯床装置2週後には,義歯床下組織に加えられた圧力によって,分画吸収面約63%,活性吸収面率約75%の値を示し,活発な骨吸収が観察された.義歯床装置3週後では,破骨細胞ほとんど消失し,骨芽細胞の出現に伴う骨の添加が観察され,分画形成面約30%,活性形成面率約30%の測定値を示した.義歯床装置4週後では分画形成面約60%,活性形成面率約70%,石灰化速度約2μm/dayと観察期間中最も高い測定値を示し,活発な骨の添加が観察されたが,以後の観察期間では骨の添加は次第に減少し,義歯床装置10週後には観察されなかった.顕微X線写真の観察においても,義歯床装置4週後から6週後に吸収窩に相当してX線透過度の高い新生骨の添加が観察された.以上のように,義歯床下の骨組織の動態は義歯装着後の短期間に生じることが明らかとなったが,現在もさらに詳細な検討を行っている.

The tissue under the free dental bed is maintained in a non-cleaning state, and the epithelial cells under the free dental bed are maintained in a non-cleaning state. The relationship between the absorption and addition of bone tissue under the mandibular implant was studied. At 12 weeks of age, the male implant was 50 and the maxillary implant was installed at 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 weeks of age. 9 days before the end of the inspection period, the time limit is 2 days before the inspection period. Villanueva's bone staining was applied to the obtained flap tissue, and the bone was ground without deliming. After the applied micro-X-ray imaging device was used for micro-X-ray photography, optical micro-mirrors, optical micro-mirrors and two-dimensional image analysis devices were used for observation and morphological measurement. After 1 week of the artificial dental device, the bone dynamics were changed. After 2 weeks, the tissue under the artificial dental bed was under pressure, the absorption area was about 63%, the active absorption area was about 75%, and the active bone absorption was observed. After 3 weeks, osteoclasts disappeared and bone bud cells appeared with bone addition. The percentage of active osteoclast formation was about 30%. After 4 weeks, the percentage of active formation was about 70%, and the lime rate was about 2μm/day. The highest value was observed during the observation period. The addition of active bone decreased gradually during the observation period. After 10 weeks, the addition of active bone decreased. Micro-X-ray photographs were taken after 4 weeks and 6 weeks of dental implant installation, and the addition of high X-ray transmittance and new bone was observed. The bone tissue dynamics under the tooth bed are discussed in detail after the tooth is installed.