高カルシウム食が実験的骨粗鬆症における顎骨の骨動態に及ぼす影響

高钙饮食对实验性骨质疏松症颌骨骨动力学的影响

• 批准号: 08672236
• 负责人: 佐藤 隆志
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08672236/
• 关键词: 骨粗鬆症; 高Ca食; 下顎骨; 硬口蓋骨組織; 骨形態計測; ラット

本研究は,骨粗鬆症における義歯床下組織の変化に関する研究の一環として,低カルシウム(Ca)食飼育と卵巣摘出とによって誘発した骨粗鬆症ラットの下顎骨骨組織および臼歯部口蓋骨組織の吸収ならびに形成動態に対して,高Ca食が及ぼす影響について組織計測的ならびに病理組織学的手法を用いて検討した.6週齢のウイスター系雌性ラット45匹×2群=90匹に両側の卵巣摘出を施し,施術12週後まで低Ca食(Ca:0.02%)飼育を行い,実験的に骨粗鬆症を発症させた.その後,低Ca食群には施術32週後に至るまで低Ca食を与え続け,高Ca食群には施術12週経過の後は高Ca食(Ca:2.30%)を与えた.観察期間は施術12,13,14,16,18,20,22,24および32週後とし,各観察期間終了の10日前と3日前に骨組織の蛍光ラベリングを行った.各観察期間毎に各実験群の5匹ずつを屠殺して,下顎骨および臼歯部口蓋骨組織を採取し,Villanuevaの骨染色を施した後,前頭断末脱灰研磨標本を作成した.二次元画像解析装置を用いて測定した第一次パラメータに基づいて,第二次パラメータとして分画吸収画率,分画形成面率ならびに石灰化速度を算出した.なお,下顎骨では海綿骨を計測の対象とした.得られた第二次パラメータについて実験群毎に平均値と標準偏差を求め,各観察期間毎に各実験群間の有意差検定(t検定)を行った.その結果,骨組織の形成と吸収は,下顎骨では観察期間を通じていずれも経時的に減少しつつほぼ平衡状態を保った.一方,臼歯部口蓋骨組織では,術後13〜16週(高Ca食飼育後1〜4週)には形成量が吸収量を上回り,術後18週以降にはほぼ平衡状態を示した.本研究の結果は,本研究の同一条件下で施術12週後以降に観察された下顎骨における骨量の維持ならびに臼歯部口蓋骨組織における骨量の増加(原ら,1996)と整合性を示すものである.

In this study, we studied the histology and histology of osteoporosis under the bed. In the first part of the study, we extracted the oocytes from the oocytes of low fat diet (Ca). The bone tissues of the molars and the mouth of the molars were absorbed to form a dynamic cycle. The histopathological techniques of the high-Ca diet and the high-Ca diet were performed by using the histopathology method of the high-Ca diet. 6-year-old female oocytes were harvested in 45 eggs × 2 groups = 90 eggs. After 12 weeks, the animals were fed with low-Ca diet (low-DNA diet) and osteosarcoma syndrome. After 32 weeks of treatment, the low-Ca diet group received low-Ca diet and diet diet, while the high-Ca diet group received high-Ca diet (Ca:2.30%) diet after 12-week treatment. During the period of observation, 1213pas 13pas 14pas 16pas 18pas 20pas 22pas 22pas 32 years later, and during each period of observation, the bone tissue was lighted 10 days ago and 3 days ago. During each observation period, 5 animals of each group were slaughtered, and the bone tissue of the molar of the lower bone was harvested. After the Villanueva bone staining was applied, the mark of ash removal and grinding at the end of the fracture was made. The two-dimensional portrait parsing device is used to determine the drawing rate of the first time, the second time, the area rate of the drawing, the rate of calming, and the rate of surface formation. Look at the bottom of the bone, the bone. It is necessary to calculate the standard deviation of the average standard for the second time, and to determine the intentional difference (t determination) among the groups during each inspection period. The results showed that the bone tissue formed a suction trap, and the bone tissue was in a state of balance during the observation period. On one side, the bone tissue of the acetabulum was loose, and then 13-16 years old (1-4 weeks after high-Ca diet) the amount of bone formation increased, and then 18 years later, the balance of the bone tissue was reduced. The results of this study are as follows: in this study, under the same conditions, after 12 years of surgery, we observed that the bone mass of the bone tissue of the molars was maintained and the bone mass of the bone tissue was increased (original, 1996).