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Search for anti-allergic constituents in South-East Asian traditional medicine

寻找东南亚传统药物中的抗过敏成分

基本信息

批准号：
03671002
负责人：
YAMASAKI Kazuo
金额：
$ 1.34万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Bioassay method of measuring inhibiting activity of histamine release from mast cells was improved to be able to measure many samples at a time rapidly.By measuring the activity of several Philippine traditional medicinal plants, the following plants (MeOH extract) which showed strong activity, were selected for further study: Ehretia microphylla, E. philippinensis, Cordia myxa (Boraginaceae). These plants were locally used for anti-allergic and anti-inflammatory purposes.From the first and the second plant extracts, rosmarinic acid was isolated and characterized by NMR analysis. This compound was known to have anti-inflammatory activity, and it may well be responsible for the effect of these herbal medicines.Again from the first plant, two novel acyclic diterpene glucosides and allantoin were isolated and the chemical structure were elucidated by instrumental analysis, especially NMR spectroscopy.The diterpene glucosides were phytol congeners. A similar compound, plaunotol was recently found from Thai medicinal plant and is now being used as an anti-ulcer medicine. From the similarity of the structure, our glucosides need to be tested for such activity. Allantoin is known to be topical vulnerary and is used for skin ulcer therapy, so it may also be a contributor to the activity of the said plant. It is to be noted that the high content of rosmarinic acid is remarkable and the two Ehretia plants are promising sources of this active compound. From an antiallergic plant, Pesudoelephantopus spicatus, two novel sesquiterpenes were isolated and the chemical structures were decided.

本文对测定肥大细胞释放组胺抑制活性的生物测定方法进行了改进，使之能快速、多样品同时测定，并通过对几种菲律宾传统药用植物的活性测定，筛选出活性较强的植物（甲醇提取物）：小叶厚叶philippinensis、Cordia myxa（紫草科）。从第一种和第二种植物提取物中分离迷迭香酸，并通过NMR分析进行表征。从第一种植物中分离得到两个新的非环状二萜苷和尿囊素，并通过核磁共振波谱分析，确定了其化学结构，二萜苷为植醇同系物。最近从泰国药用植物中发现了一种类似的化合物，plaunotol，现在被用作抗溃疡药。从结构的相似性来看，我们的糖苷需要测试这种活性。已知尿囊素是局部药物，用于皮肤溃疡治疗，因此它也可能是所述植物活性的贡献者。值得注意的是，迷迭香酸的高含量是显著的，并且两种Ehretia植物是这种活性化合物的有希望的来源。从抗过敏植物穗花假地胆草中分离得到两个新的倍半萜类化合物，并确定了其结构。