Proteomic and Transcriptional Profiling of Cartilage

软骨的蛋白质组学和转录分析

• 批准号: DP0343693
• 负责人: Prof John Bateman
• 金额: $ 18.85万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-07 至 2005-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0343693
• 关键词: Proteomic; Transcriptional; Profiling; Cartilage

Gene expression and signalling pathways that regulate cartilage formation, and its orderly transition to bone, are poorly described. Our studies will, for the first time, combine two complementary cutting-edge approaches, protein identification by proteomic analysis, and mRNA profiling by microarray analysis, to define these pathways and develop a comprehensive catalogue of proteins and gene expression patterns during cartilage development and bone formation. This information will provide insight into the regulation of cartilage differentiation, maturation and structure, and will provide a critical platform for the development of more sophisticated cartilage and bone biomaterials for improved tissue repair and regeneration.

调节软骨形成的基因表达和信号通路，以及软骨向骨的有序过渡，目前还没有得到很好的描述。我们的研究将首次结合两种互补的前沿方法，即蛋白质组学分析的蛋白质鉴定和微阵列分析的mRNA分析，来定义这些途径，并开发软骨发育和骨形成过程中蛋白质和基因表达模式的综合目录。这一信息将为软骨分化、成熟和结构的调控提供洞见，并将为开发更复杂的软骨和骨生物材料提供关键平台，以改善组织修复和再生。