Estimation of plasma dopamine concentration and its clinical significance

血浆多巴胺浓度的估算及其临床意义

• 批准号: 03671111
• 负责人: YOSHIMURA Manabu
• 金额: $ 1.34万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03671111/
• 关键词: dopamine; catecholamine; HPLC; exercise; hypertension; conjective heart failure; parkinsonisms; laboratory medicine; ド-パミン; 遊離型と抱合型; 脱抱合; 高血圧症; 副腎機能異常症

The majority of circulating catecholamines (CAs) is in a sulfoconjugated form in human plasma and the plasma free dopamine (DA) concentration was the detection limit of other high-performance liquid chromatography (HPLC) techniques. Using diphenylethylenediamine derivatives for fluorogenic reagent, it is possible to estimate free DA concentration as little as 0.03 pmol/ml in HPLC analysis. The valves of free DA obtained from healthy volunteers were 6.4 * 1.5pg/ml in young subjects and 16.2 * 8.2pg/ml in aged subjects, which indicated the effect of age on plasma DA concentration. Plasma DA level increased in sitting and standing position. During 24 hours, plasma DA level did not change. During exercise, plasma DA level increased with the enhanced intensity of exercise and showed the positive correlation with plasma lactic acid level, indicating vasodilating effects on the vessels at muscle and skin. Plasma DA concentration increased in patients with pheochromocytoma, primary aldorterorism, congestive heart failure and L-DOPA treatment of parkinsonisms. The estimation of plasma DA concentration can be applied for differential diagnosis in laboratory medicine

大多数循环中的儿茶酚胺（CA）在人血浆中以磺基共轭形式存在，血浆游离多巴胺（DA）浓度是其他高效液相色谱（HPLC）技术的检测限。使用二苯基乙二胺衍生物的荧光试剂，它是可能的估计游离DA浓度低至0.03 pmol/ml的HPLC分析。健康志愿者血浆游离DA值青年组为6.4 × 1.5pg/ml，老年组为16.2 × 8.2pg/ml，表明年龄对血浆DA浓度有影响。血浆DA水平在坐位和站立位时升高。在24小时内，血浆DA水平没有变化。运动时，血浆DA水平随运动强度的增加而升高，并与血浆乳酸水平呈正相关，表明运动对肌肉和皮肤血管有舒张作用。嗜铬细胞瘤、原发性Aldorterorism、充血性心力衰竭和L-DOPA治疗的帕金森病患者血浆DA浓度升高。血浆DA浓度的测定可应用于实验室医学的鉴别诊断

项目成果

M.YOSHIMURA et al.: "Advances in Biosciences" Pergamon Press,LTD, 11 (1993)

M.YOSHIMURA 等人：《生物科学进展》Pergamon Press,LTD, 11 (1993)

Manabu YOSHIMURA, et al.: "Role of plasma dopamine for regulation of blood pressure in humans" Advances in Biosciences Pergamon Press, LTD.

Manabu YOSHIMURA 等人：“血浆多巴胺对调节人类血压的作用”生物科学进展 Pergamon Press, LTD。