Survey of intestinal disaccaridase activities in the wild insectivores and development of disease model for disaccaridase deficiency

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1)Intestinal disaccaridase activities were survey in the wild insectivores to develop animal models for human disaccaridase deficiency. Lack of sucrase activities was detected in some insectivore colonies but oher enzyme activities (isomaltase, maltase, and treharase) were present.2)Suncus murinus in Taramajima-island were lack of sucrase activity although all suncus murinus in Sri Lanka, Bangladesh, Okinawa-island and Tokunoshima-island have enzyme activities. We established TR,NAG and MI strains with sucrase deficiency in suncus murimus.3)Lack of sucrase activities in the intestinal brush border membranes was found in Japanese wild Soricinae species such as Sorex caecutiens (1 animal in 1 area), Sorex gracillimus (8 animals in 1 area), Sorex hosonoi (2 animals in 1 area), Sorex sadonis (4 animals in 1 area), Sorex shintto (5 animals in 2 areas), and Sorex unguiculatus (7 animals 1 area). We also found lack of sucrase activities in Chimalogale himalayica (2 animals in 1 area) and Anourosorex squamipes (3 animals in 1 area).4)All disaccaridase activities were detected in Crocidura dsinezumi (4 animals in 2 area), Mogera kobeae (6 animals in 1 area), Mogera wogura (1 animal in 1 areae, Urotrichus talpoides (12 animals in 6 areas) and Dymecodon pilirostris (12 animals in 2 areas).