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Developmental Studies of Image Analizing System for Animal Karyotype

动物核型图像分析系统的开发研究

基本信息

批准号：
07508006
负责人：
ODA Sen-ichi
金额：
$ 5.5万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07508006/
关键词：
Image analyzing Karyotype Chromosome Banding pattern 画像処理カリオビジョン核型

项目摘要

The automation of chromosome analysis is a classific example of contexed -conditioned pattern recognition. During the last years, several systems have been developed to enable automatic karyotyping of human chromosomes. We developed the application software of a image analyzing system for animal karyotypes based on one of these systems for human one, the Karyovision CA2000K from The Sumitomo Metal Co Ltd. Developmental studies were carried out during 3 years as following : 1) We built our net work of computer machine for development of image analyzing system for animal karyotype. We changed a managing sheet or data sheet from for human to animal. In that case it is important to record species' name, collector' name, location of collection and date of collection in wild animals, and species' name, strain's name, and holder or breeder's name in laboratory animals. 2) Using our image analyzing system, we applied many species following as ; Karyograms of Chinese hamsters and house musk shrews, which have all types of chromosomes such as human were recognized well. We also input and analize domestric animals such as pig, goat and cattle, and wild animals such as field vole, shrew-mole so on. It is difficult to recognize karyograms of goat and cattle. Recognition frequencies were between 94,5% in chinese hamster and 67,55 in cattle. 3) We stored freezing cells from animals to supply and to analyze these karyotypes. Using these samples we can develop a system with a better recognizing abilities. We also established standard karyotypes of these animals by banding methods of chromosomes, and we developed new strains with chromosomal deviations, such as translocations and inversion in the Chinese hamster and Robertsonean translocation in the shrew. 4) We took digital images of chromosomes to analyze directly and automatically through a microscope.

染色体分析的自动化是上下文条件模式识别的一个典型例子.在过去的几年中，已经开发了几个系统，使人类染色体的自动核型分析。本课题组以日本住友金属株式会社的Karyovision CA 2000K为基础，开发了动物核型图像分析系统的应用软件。我们将管理表或数据表从人类改为动物。在这种情况下，记录野生动物的种名、采集者姓名、采集地点和采集日期，以及实验动物的种名、品系名称和保持器或饲养员姓名是很重要的。2)利用我们的图像分析系统，我们应用于以下几个物种：中国仓鼠和家麝鼩，这是所有类型的染色体，如人类的染色体核型识别良好。对猪、羊、牛等家养动物和田鼠、地鼠等野生动物的染色体组型进行了输入和分析。识别频率在中国仓鼠中为94.5%，在牛中为67.55%。3)我们储存了动物的冷冻细胞，以提供和分析这些核型。利用这些样本，我们可以开发一个系统，具有更好的识别能力。我们还建立了这些动物的标准核型的染色体显带方法，我们开发了新的菌株与染色体偏差，如易位和倒位的中国仓鼠和罗伯逊易位的鼩。4)我们拍摄了染色体的数字图像，通过显微镜直接自动分析。