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Characterizaion and evaluation of soricid mammals : Basic studies on their domestication as unique laboratory animals

群科哺乳动物的特征和评价：作为独特实验动物的驯化基础研究

基本信息

批准号：
06044100
负责人：
ODA Sen-ichi
金额：
$ 3.65万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044100/
关键词：
Shrew Sorex Insectivora Laboratory Animal Russia Genetic Resources

项目摘要

1) We collected 124 insectivores such as Sorex araneus, Sorex caecutiens, Sorex minutus, Sorex isodon, Sorex tundrensis, Neomys fodiens, Asioscalops altaica and Sorex spp.at the field in Novosibirk, Siberia, Russia on July and Aug.1996, and compared with the Japanese shrews.2) The common shrew, Sorex araneus is subdivided into many chromosomal races distinguished by Robertsonian rearrangements. The Novosibirsk race has combination : hn, go, ik, mp. The house musk shrew, Suncus murinus also shows Robertsonian chromosomal variations from chromosomal number 2n=30 to 40. We established the standard karyotype and each Robertsonian fusions of Suncus murinus by the high resolution banding method. Theses two species, Sorex araneus and Suncus murinus are good animal models to estimate the effects of Robertsonian heterozygosity on reproductive performance.3) To study the phylogenic relationships between Sorex species, the nuclear ribosomal DNA spacer and mitochondrial cytochrome b nucleotide seq … More uences were analyzed on Sorex shinto collected in Honshu, Japan and Sorex caecutincecollected in Siberia, Russia. In previous report, these shrews were included in one species, but our results suggested that it is reasonable for the two animals to be divided into distinct species.4) We tried to search for animals with lack of dissacharidase activities. We found that no activities of sucrase were showed in Soricinae shrew such as Sorex araneus, Sorex caecutiens, Sorex minutus, Sorex isodon, Sorex tundrensis, Neomys fodiens collected in Russia and Sorex shinto, Sorex saevus, Sorex sadonis, Sorex unguiculatus collected in Japan, and that presence of sucrase activities were showed in Crocidurinae shrews such as Crocidura sibirica collected in Russia and Crocidura dsinezumi and Suncus murinus except Suncus murinus collected in Nagasaki and Tarama-island, Japan. Lack of sucrase activities in Suncus murinus was controlled by an autosomal single gene. Our results indicates that these animals with no activities of sucrase are potent as an animal model for human disease. Less

1)1996年7月至8月在俄罗斯西伯利亚新西伯利亚野外采集了124种食虫动物，包括：蜘蛛Sorex araneus、囊囊蜘蛛Sorex caecutiens、微小Sorex minutus、等齿Sorex isodon、通化Sorex tundrensis、新鼠Neomys fodiens、阿尔泰Asioscalops altaica和小鼩 Sorex spp.at，并与日本鼩进行了比较。新西伯利亚比赛有组合：hn，go，ik，mp。家麝鼩（Suncus murinus）也显示罗伯逊染色体变异，染色体数目为2n=30至40。采用高分辨分带法建立了臭鼩的标准核型和各罗伯逊融合体。3）研究了梭鱼属物种与核核糖体DNA间隔区和线粒体细胞色素b核苷酸序列之间的遗传关系，并对梭鱼属物种与核核糖体DNA间隔区和线粒体细胞色素b核苷酸序列之间的遗传关系进行了初步探讨，结果表明，梭鱼属物种与核核糖体DNA间隔区和线粒体细胞色素b核苷酸序列之间的遗传关系， ...更多信息本文对日本本州采集的鱼和俄罗斯西伯利亚采集的鱼进行了基因组学分析。在以前的报道中，这两种动物被归为一个种，但我们的结果表明，这两种动物被分为不同的种是合理的。4）我们试图寻找缺乏dissacharidase活性的动物。结果发现，在俄罗斯采集的蜘蛛Sorex araneus、囊囊囊Sorex caecutiens、微小Sorex minutus、等齿Sorex isodon、通化Sorex tundrensis、南方新鼠Neomys fodiens和日本采集的新藤Sorex shinto、萨氏Sorex saevus、萨氏Sorex sadonis、长爪Sorex nguiculatus等中，除俄罗斯产的西伯利亚大鳄鱼Crocidura sibirica、大鳄鱼Crocidura dsinezumi和臭鼩 Funcus murinus外，日本长崎和多良间岛采集的臭海鲶。梭鱼蔗糖酶活性的缺失受一个常染色体单基因控制。我们的研究结果表明，这些动物没有活性的蔗糖酶是有效的动物模型，为人类疾病。少