The house musk shrew, Suncus murinus as unique laboratory animal model for estimation of thyroid hormone function

麝香鼩（Suncus murinus）作为评估甲状腺激素功能的独特实验动物模型

• 批准号: 14580797
• 负责人: ODA Sen-ichi
• 金额: $ 2.37万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580797/
• 关键词: Suncus; house musk shrew; type-1 iodothyronine deiodinase (D1); laboratory animal; thyroid hormone; type-2 iodothyronine deiodinase (D2); fasting; brown adipose tissue; トガリネズミ; 脱ヨード酵素; 脱ヨード酵素遺伝子(D1); 脱ヨード酵素遺伝子(D2); 遺伝子クローニング

1) The house musk shrew Suncus murinus belongs to the most primitive mammalian oder Insectivora. This species is widely distributed in hot climate area from East Africa to East Asia2) We found that the type-1 iodothyronine deiodinase (D1) activity in shrew liver was one third of that in the rat liver. The other hand, D2 activity was higher level. To elucidate the mechanism for those activities, we cloned the D1 and D2 cDNA from the shrew liver. RT-PCR was performed using different sets of primers, which were designed based on conserved amino acid sequences of the D1, D2 and D3 among different species. The cloned cDNAs contain the entire open reading frame. A putative selenocystein (Sec) was identified, which is conserved among mammalian species. Cloned the D1 and D2 cDNA from the shrew were comparable same to other mammal species3) Blood T3 level in the shrew was stable at the fasting status, but T3 level in the mouse and rat was decreased. D1 mRNA in the liver and D2 mRNA in the brown adipose tissue were reduced but D2 activities in the brain were incresed. Brain D2 activities may support normal blood T3 level in the shrew4) Blood T3 level in the shrew was constant in fasting status and basic metabolism was not reduced in fasting status. So, the shrew is soon died in fasting status. The shrew is so unique animal to study the mechanism of thyroid hormone metabolism comparable to the rats and mice

1)家麝鼩（Suncus murinus）属于食虫目（Insectivora）中最原始的哺乳动物。2）发现鼩 鼱肝中Ⅰ型碘甲腺原氨酸脱碘酶（D_1）活性仅为大鼠肝的1/3。D2活性较高。为了阐明这些活动的机制，我们克隆了D1和D2的cDNA从鼩肝脏。根据D1、D2和D3在不同物种中的保守氨基酸序列设计引物，进行RT-PCR。克隆的cDNA包含整个开放阅读框架。一个推定的硒半胱氨酸（Sec）的鉴定，这是保守的哺乳动物物种。3）在禁食状态下，鼩的血T_3水平稳定，而小鼠和大鼠的血T_3水平下降。肝脏中D1 mRNA和棕色脂肪组织中D2 mRNA降低，而脑中D2活性升高。脑D2活性可能支持正常血T3水平。4）禁食状态下，血T3水平稳定，基础代谢水平未降低。所以，这只鼩 鼱很快就在禁食状态下死去。与大鼠和小鼠相比，鼩是研究甲状腺激素代谢机制的独特动物

项目成果

ROGATCHEVA Margarita, HAYASHI Yoshitaka, ODA Senichi, et al.: "Tvpe1 iodothvronine deioclinase in the house musk shrew (Suncus murinus, Insectivora : Soricidae) : cloning and characterization of complementary DNA, unique tissue distribution and regulation

ROGATCHEVA Margarita、HAYASHI Yoshitaka、ODA Senichi 等人：“家麝鼩（Suncus murinus，食虫目：Soricidae）中的 Tvpe1 碘代膦脱碘酶：互补 DNA 的克隆和表征、独特的组织分布和调节

SUZUKI Daisuke., TAKEUCHI Yoko., ODA Sen-ichi, MURATA Yoshiharu: "Cloning of a cDNA for type II iodothyronine 5 deiodinase in the house musk shrew (Suncus murinus, Insectivora : Soricidae)"Environmental Medicine. 46(1). 62-65 (2002)

SUZUKI Daisuke.、TAKEUCHI Yoko.、ODA Sen-ichi、MURATA Yoshiharu：“在家麝鼩（Suncus murinus，昆虫纲：Soricidae）中克隆 II 型碘甲腺原氨酸 5 脱碘酶 cDNA”环境医学。