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MAPPING AND DEVELOPMENTAL BIOTECHNOLOGY OF THE ter GENE RESPONSIBLE FOR GERM CELL DEFICIENCY IN MICE.

小鼠生殖细胞缺陷的 ter 基因的定位和开发生物技术。

基本信息

批准号：
03680037
负责人：
NOGUCHI Motoko
金额：
$ 1.34万
依托单位：
SHIZUOKA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680037/
关键词：
The ter(teratoma) gene Sterility Germ cell deficiency Primordial germ cell Reconstituted testis Mouse Gene mapping Developmental biotechnology 精巣性テラトーマ teratoma(ter)terコンジェニック系統再構成生殖巣

项目摘要

The ter (teratoma) gene causes germ cell deficiency in 129/Sv-ter strain of mouse and the ter-congenic strains, C57BL/6J-ter and LTXBJ-ter which we established by introduction of the ter gene from 129/Sv-ter mice onto the genetic background of C57BL/6J and LTXBJ strains.Mapping and developmental biotechnological method such as reconstituted testes served as useful tools in studies on the mechanism of sterility induced by the ter gene as summarized below.1. Counts of Alkaline phosphatase positive cells considered to be primordial germ cells (PGCs) per embryo showed that the ter gene causes deficiency of PGCs in their migration stages in ter/ter fetuses in LTXBJ-ter strain, whereas PGCs in +/+ and +/ter fetuses proliferate as well as those in LTXBJ strain.2. In testes reconstituted from reaggregates of PGCs and somatic cells from dissociated hindgut and fetal testes in 129/Sv-ter(+/+) mice that is susceptible to testicular teratomas, PGCs from hindgut differentiated to normal gametes and teratomas, suggesting that reconstituted testes can also serve as useful tools in analysis of the ter gene function in PGC migration stages.3. The linkage tests performed between the ter gene and 32 genetic markers using C57BL/6J-ter (+/ter) mice and several inbred strains showed that the ter gene maps closely to Grl-1 locus on mouse Chromosome 18 and that the ter genotype of each embryo or adult can be identified by PCR polymorphisms of the Grl-1 gene.4. Fetal testes in LTXBJ-ter strain were dissociated and germ cells (+/+ and +/ter) were reaggregated with somatic cells (+/+ and +/ter) or (ter/ter). Grafts of reaggregate in the former combination reconstituted normal testes, but those in the latter combination did germ cell deficient testes. It is suggested that the ter gene is expressed on testicular somatic cells and resultant but unknown defect induces in turn germ cell deficiency.

的ter（畸胎瘤）基因导致129/Sv-ter品系小鼠和ter-congenic品系中的生殖细胞缺陷，C57 BL/6 J-ter和LTXBJ-ter是我们通过将129/Sv-ter小鼠的ter基因导入C57 BL/6 J-ter的遗传背景而建立的。遗传作图和发育生物技术方法如重组睾丸是研究不育机制的有效工具如下所述由ter基因诱导。对每个胚胎中碱性磷酸酶阳性细胞（被认为是原始生殖细胞（PGCs））的计数表明，在LTXBJ-ter品系的ter/ter胎儿中，ter基因导致PGCs在其迁移阶段的缺陷，而在+/+和+/ter胎儿中，PGCs与LTXBJ品系的PGCs一样增殖.在睾丸畸胎瘤易感小鼠129/Sv-ter（+/+）中，后肠PGCs和胎儿睾丸分离的PGCs与体细胞重组后的睾丸中，后肠PGCs分化为正常配子和畸胎瘤，提示重组睾丸也可作为分析PGC迁移阶段ter基因功能的有用工具.利用C57 BL/6 J-ter（+/ter）小鼠和几个近交系进行的ter基因与32个遗传标记的连锁检测表明，ter基因与小鼠18号染色体上的Grl-1位点紧密连锁，每个胚胎或成体的ter基因型都可以通过Grl-1基因的PCR多态性来鉴定.将LTXBJ-ter品系中的胎儿睾丸分离，生殖细胞（+/+和+/ter）与体细胞（+/+和+/ter）或（ter/ter）重新聚集。前一种组合中的重组体移植物重建了正常睾丸，而后一种组合中的重组体移植物重建了生殖细胞缺陷的睾丸。这表明ter基因在睾丸体细胞上表达，由此产生的但未知的缺陷反过来诱导生殖细胞缺陷。