CELL BIOLOGICAL AND BIOCHEMICAL STUDIES ON FUNCTION OF THE ter GENE IN PRIMORDIAL GERM CELL DEFICIENCY IN ter MUTANT MICE.

具有ter （teratoma, Chr.18）突变的突变株可作为研究小鼠原始生殖细胞（PGC）增殖、存活和致畸癌变的有用工具。得到的ter基因的特征总结如下：1。短句来源本研究通过将129/Sv-+/ter小鼠的ter基因引入到B6、LTXBJ和C3H菌株的遗传背景中，建立了从妊娠第8天起，B6-+/ter、LTXBJ-+/ter和C3H-+/ter的ter基因诱导PGC缺乏症。3.利用三基因座附近微卫星标记的SSLP鉴定各三基因型。突变体睾丸中PGC缺失是由于小管内缺陷造成的，有睾丸体细胞的重组睾丸中+/+性腺细胞发生凋亡，而有自身体细胞的重组睾丸中+/+性腺细胞分化为生精细胞。这表明ter基因可能在其体细胞中起作用。这种类型的PGC缺乏症不能通过在体外添加几种PGC生长因子来挽救，并在PGC的G1期被细胞凋亡所诱导。与自身体细胞共培养的PGC在胚胎性腺+/+和+/ter体细胞的条件培养基（CM）中增殖，而在条件培养基（CM）中增殖不明显。+/+CM含有支持PGC存活的蛋白样物质，而terCM不含。5 . +/+和+/+分离的PGC均在+/+体细胞上增殖，而在+/+体细胞上发生凋亡。结论：性腺体细胞可能产生一种支持PGC存活的新型生长因子（称为TER因子，TERF），包括可溶性型和膜结合型，该因子可能由TER位点上的正常基因编码，其PGC是正常的。

Mutant strains having the ter (teratoma, Chr.18) mutation can serve as useful tools for studies of proliferation, survival and teratocarcinogenesis of primordial germ cells (PGC) in mice. Characteristics of the ter gene obtained are summarized below :1.The ter gene induces PGC deficiency from 8 days of gestation in ter/ter mice of the ter-congenic strains such as B6-+/ter, LTXBJ-+/ter and C3H-+/ter which we have established by introducing the ter gene from 129/Sv-+/ter mice onto the genetic backgrounds of B6, LTXBJ and C3H strains, respectively.2.Each ter genotype of each embryo of ter-congenic strains was identified by SSLP of the microsatellite markers near the ter locus.3.PGC deficiency in ter mutant testes is ascribed to their intratubular defect and +/+ gonocytes occurred apoptosis in reconstituted testes with ter testicular somatic cells, whereas they differentiated to spermatogenic cells in those with own somatic cells. These indicate that the ter gene may function in ter somatic cells.4.This type PGC-deficiency was not rescued by addition of several PGC growth factors in vitro and was induced by apoptosis in the G1 phase of PGC.5.PGC co-cultured with own somatic cells proliferated in conditioned medium (CM) of +/+ and +/ter somatic cells of fetal gonads, but they did not in terCM.+/+CM contained protein like substance supporting PGC survival, but terCM did not. Both +/+ and ter PGC isolated proliferated on the +/+ somatic cells, but they occurred apoptosis on the ter ones.6.It is concluded that a novel PGC growth factor (designated as TER Factor, TERF), both soluble type and membrane bounded type, supporting PGC survival may be produced by gonadal somatic cells and that it may be coded by the normal gene on the ter locus, and also that ter PGC are normal.